+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Immunohistochemical staining with cytokeratin combining semi-serial sections for detection of cervical lymph node metastases of oral squamous cell carcinoma



Immunohistochemical staining with cytokeratin combining semi-serial sections for detection of cervical lymph node metastases of oral squamous cell carcinoma



Auris Nasus Larynx 34(3): 347-351



Lymphatic metastatic characteristics of oral squamous cell carcinoma are not fully understood, for instance, skip metastasis is still controversial. The purposes of the present study was to explore the accuracy and applicability of immunohistochemical stain with cytokeratin combining semi-serial sections for detection of cervical lymph node metastasis of oral squamous cell carcinoma. Regional lymph nodes (N=1638) were obtained from 26 patients with primary oral squamous cell carcinoma who underwent five level neck dissections. Semi-serial sections at an interval of 0.5mm was performed for each lymph node and cross-detected by immunohistochemical staining with cytokeratin and traditional hematoxylin-eosin staining (H-E) and their accuracies were compared. Of 26 patients, 21 were detected having lymphatic metastasis by H-E staining and 26 by immunohistochemical detection; Of 1638 lymph nodes, 52 metastatic lymph nodes were detected by H-E staining while 162 by immunohistochemical detection. One case with cancer of the mouth floor being defined having skip metastasis was proved having no skip metastasis by the immunohistochemical detection. The immunohistochemical detection method with semi-serial sections has higher accuracy than the traditional H-E staining and its application may present a need to re-evaluate the neck metastatic patterns of oral squamous cell carcinoma.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053703462

Download citation: RISBibTeXText

PMID: 17399927

DOI: 10.1016/j.anl.2006.12.001


Related references

Comparing the efficacy of routine H&E staining and cytokeratin immunohistochemical staining in detection of micro-metastasis on serial sections of dye-mapped sentinel lymph nodes in colorectal carcinoma. Advanced Biomedical Research 5: 13, 2016

O85. The role of Ct, Mr, Us, and 18F-Fdg Pet/Ct in detection of upper cervical lymph node metastases in oral cavity squamous cell carcinoma. Oral Oncology Suppl. 3(1): 84-85, 2009

Coordinate expression of cytokeratin 8 and cytokeratin 17 immunohistochemical staining in cervical intraepithelial neoplasia and cervical squamous cell carcinoma: an immunohistochemical analysis and review of the literature. Gynecologic Oncology 108(3): 598-602, 2008

Detection of cervical lymph node micrometastasis and isolated tumor cells in oral squamous cell carcinoma using immunohistochemistry and serial sectioning. Journal of Oral and Maxillofacial Pathology 20(3): 436-444, 2016

Analyses of cervical lymph node metastases in oral squamous cell carcinoma. European Journal of Cancer 35(Suppl. 4): S174, 1999

Detailed topography of cervical lymph-node metastases from oral squamous cell carcinoma. International Journal Of Oral & Maxillofacial Surgery. 26(1): 3-9, 1997

The use of an immunohistochemical diagnostic panel to determine the primary site of cervical lymph node metastases of occult squamous cell carcinoma. Human Pathology 41(3): 431-437, 2010

Effectiveness of immunohistochemical techniques in the detection of lymph node micrometastases of cervical squamous cell carcinoma. International Journal of Gynecological Cancer 1(1): 33-36, 1991

18F-Fluorodeoxyglucose Positron Emission Tomographycomputed Tomography for Diagnosis of Cervical Lymph Node Metastases of Oral Squamous Cell Carcinoma. Asian Journal of Oral and Maxillofacial Surgery 21(3-4): 88-95, 2009

Morphological MRI criteria improve the detection of lymph node metastases in head and neck squamous cell carcinoma: multivariate logistic regression analysis of MRI features of cervical lymph nodes. European Radiology 19(3): 626-633, 2009

The role of ultrasound in the detection of cervical lymph node metastases in clinically N0 squamous cell carcinoma of the head and neck. Cancer Imaging 7: 167-178, 2007

Lymph node and pulmonary metastases after transplantation of oral squamous cell carcinoma cell line (HSC-3) into the subcutaneous tissue of nude mouse: detection of metastases by genetic methods using beta-globin and mutant p53 genes. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology 105(4): 486-490, 2008

Lymph node and pulmonary metastases after transplantation of oral squamous cell carcinoma cell line (HSC-3) into the subcutaneous tissue of nude mouse: detection of metastases by genetic methods using beta-globin and mutant p53 genes. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics 105(4): 486-490, 2008

Patterns of cervical lymph node metastases in oral tongue squamous cell carcinoma: implications for elective and therapeutic neck dissection. Journal of Laryngology and Otology 128(3): 268-273, 2014

BerEp4, cytokeratin 14, and cytokeratin 17 immunohistochemical staining aid in differentiation of basaloid squamous cell carcinoma from basal cell carcinoma with squamous metaplasia. Archives of Pathology and Laboratory Medicine 137(11): 1591-1598, 2013