In situ hybridization with labeled probes: assessment of african Swine Fever virus in formalin-fixed paraffin-embedded tissues

Ballester, M.; Rodríguez, F.

Methods in Molecular Biology 1247: 209-218

2015


ISSN/ISBN: 1940-6029
PMID: 25399099
DOI: 10.1007/978-1-4939-2004-4_16
Accession: 053765807

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Abstract
In situ hybridization (ISH) has become a very valuable molecular diagnostic tool to detect specific DNA or RNA sequences in biological samples through the use of complementary DNA- or RNA-labeled probes. Here, we describe an optimized in situ hybridization protocol to detect African swine fever virus (ASFV) DNA in formalin-fixed, paraffin-embedded tissues using digoxigenin-labeled probes.

In situ hybridization with labeled probes: assessment of african Swine Fever virus in formalin-fixed paraffin-embedded tissues