Home
  >  
Section 54
  >  
Chapter 53,775

In vitro malignant transformation of human bronchial epithelial cells induced by benzo (a) pyrene

Zhao, P.; Fu, J.; Yao, B.; Song, Y.; Mi, L.; Li, Z.; Shang, L.; Hao, W.; Zhou, Z.

Toxicology in Vitro An International Journal Published in Association with Bibra 26(2): 362-368

2012

ISSN/ISBN: 0887-2333
PMID: 22227536
DOI: 10.1016/j.tiv.2011.12.013
Accession: 053774994
Download citation:  
Text
  |  
BibTeX
  |  
RIS
In this study, the human bronchial epithelial cells (16HBE) were treated five times with 1 ?M benzo(a)pyrene (BaP), followed by 2 passages culture, and thein vitroBaP-induced malignant transformation of 16HBE cells was established. Five colonies in soft agarose were then amplified and donated as T-16HBE-C1?5 cells respectively. T-16HBE-C1?5 cells can form tumors subcutaneously in nude mice. Histopathological changes in the tumors indicated nests growth, high nuclear-cytoplasmic ratios, coarse and clumped chromatin, numerous and distinctly atypical mitoses, cell necrosis and surrounding normal adipose, muscle and connective tissue immersed. In addition, lung metastasis was observed in nude mice in T-16HBE-C1, 3 and 4 groups.In vitrocell migration assay results indicated that T-16HBE-C2?5 cells showed much lower migration capabilities than 16HBE cells. Western blotting analysis showed that the expressions of p53 and p-Akt (Ser473) in T-16HBE-C1?5 cells were significant higher than those in 16HBE cells. Our results demonstrated that BaP could induce the malignant transformation of 16HBE cells, and p53 and p-Akt (Ser473) might play crucial roles in BaP-induced carcinogenesis. The five monoclonal cell lines (T-16HBE-C1?5) with different migration capabilities could be used as research models for further understanding the mechanisms of BaP-induced carcinogenesis and cell migration.BaP-transformed 16HBE cells were established and identified by soft agarose assay. Five colonies on agarose were amplified and donated as T-16HBE-C1?5 cells. T-16HBE-C1?5 cells all formed tumors subcutaneously in nude mice. T-16HBE-C2?5 cells showed lower migration capabilities than 16HBE cells. p53 and p-Akt (Ser473) expressed at high levels in T-16HBE-C1?5 cells..

PDF emailed within 0-6 h: $19.90




Related References

Jiang, Y.; Chen, J.; Chen, X. 2001: Malignant transformation of human bronchial epithelial cells induced by benzo(a)pyrene metabolite dihydroxyepoxy benzo pyrene Wei Sheng Yan Jiu 30(3): 129-131
Han, Z.; Zhang, Y.; Xu, Y.; Ji, J.; Xu, W.; Zhao, Y.; Luo, F.; Wang, B.; Bian, Q.; Liu, Q. 2014: Cell cycle changes mediated by the p53/miR-34c axis are involved in the malignant transformation of human bronchial epithelial cells by benzo[a]pyrene Toxicology Letters 225(2): 275-284
Xing, X.; Liu, C.; Tang, S.; Li, D.; Chen, L.; Pang, Y.; Wang, Q.; Zhang, B.; Zeng, X.; Chen, W.; Xiao, Y. 2012: DNA repair gene deficiency does not predispose human bronchial epithelial cells to benzo(a)pyrene-induced cell transformation Toxicology in Vitro: An International Journal Published in Association with Bibra 26(4): 579-584
Fields, W.R.; Desiderio, J.G.; Leonard, R.M.; Burger, E.E.; Brown, B.G.; Doolittle, D.J. 2004: Differential c-myc expression profiles in normal human bronchial epithelial cells following treatment with benzo[a]pyrene, benzo[a]pyrene-4,5 epoxide, and benzo[a]pyrene-7,8-9,10 diol epoxide Molecular Carcinogenesis 40(2): 79-89
Teel, R.W.; Babcock, M.S.; Dixit, R.; Stoner, G.D. 1986: Ellagic acid toxicity and interaction with benzo[a]pyrene and benzo[a]pyrene 7,8-dihydrodiol in human bronchial epithelial cells Cell Biology and Toxicology 2(1): 53-62
Fields, W.R.; Desiderio, J.G.; Brown, B.G.; Burger, E.E.; Hellmann, G.M.; Doolittle, D.J. 2000: Differential gene expression profiles in normal human bronchial epithelial cells following benzo- pyrene and benzo pyrene diol epoxide treatment Proceedings of the American Association for Cancer Research 41: 572
Xia, B.; Yang, L.-Q.; Huang, H.-Y.; Pang, L.; Yang, X.-F.; Yi, Y.-J.; Ren, X.-H.; Li, J.; Zhuang, Z.-X.; Liu, J.-J. 2016: Repression of Biotin-Related Proteins by Benzo[a]Pyrene-Induced Epigenetic Modifications in Human Bronchial Epithelial Cells International Journal of Toxicology 35(3): 336-343
Zhang, L.; Bao, Y.; Li, J. 2011: Nuclear respiratory factor-1 is involved in mitochondrial dysfunction induced by benzo(a)pyrene in human bronchial epithelial cells Basic and Clinical Pharmacology and Toxicology 109(2): 115-122
Chen, W.; Padilla, M.T.; Xu, X.; Desai, D.; Krzeminski, J.; Amin, S.; Lin, Y. 2016: Quercetin inhibits multiple pathways involved in interleukin 6 secretion from human lung fibroblasts and activity in bronchial epithelial cell transformation induced by benzo[a]pyrene diol epoxide Molecular Carcinogenesis 55(11): 1858-1866
Yao, S.; Chen, X.; Hu, N.; Zhang, N.; Qiu, M.; Jia, Y.; Zhang, H.; Liang, J.; Chen, Z.; Zheng, L.; Zhu, J.; Mao, R.; Jiang, Y. 2023: Benzo[a]pyrene-induced up-regulation of circ_0003552 via ALKBH5-mediated m6A modification promotes DNA damage in human bronchial epithelial cells Environmental Pollution 336: 122367
Liu, L.; Jiang, Y.; Zhang, H.; Greenlee, A.R.; Yu, R.; Yang, Q. 2010: MiR-22 functions as a micro-oncogene in transformed human bronchial epithelial cells induced by anti-benzo[a]pyrene-7,8-diol-9,10-epoxide Toxicology in Vitro: An International Journal Published in Association with Bibra 24(4): 1168-1175
Spyres, L.; Wang, A.; Powell, K.L.; Yandle, K.; Mistry, H.; Liburd, N.; Bedford, E.; High, S.; Gaddis, S.S.; Macleod, M.C. 2003: Possible involvement of BTG2 in the response of normal human bronchial epithelial cells to DNA damage induced by benzo pyrene diol epoxide Proceedings of the American Association for Cancer Research 44: 1053
Liu, A.; Li, X.; Hao, Z.; Cao, J.; Li, H.; Sun, M.; Zhang, Z.; Liang, R.; Zhang, H. 2022: Alterations of DNA methylation and mRNA levels of CYP1A1, GSTP1, and GSTM1 in human bronchial epithelial cells induced by benzo[a]pyrene Toxicology and Industrial Health 38(3): 127-138
Jiang, Y.; Chen, J.; Chen, X.; Feng, S.; Yi, F. 2002: Cloning of differentially expressed cDNA sequences involved in malignant transformation induced by benzo(a)pyrene metabolite dihydroxyepoxy benzo pyrene Zhonghua Zhong Liu Za Zhi 24(3): 239-242
Jiang-Yiguo; Chen-Jiakun; Chen-Xuemin; Feng-Sumei; Yi-Fei 2002: Cloning of differentially expressed cDNA sequences involved in malignant transformation induced by benzo (a) pyrene metabolite dihydroxyepoxy benzo pyrene Zhonghua Zhongliu Zazhi 24(3): 239-242
Qiu, M.; Zhang, N.; Yao, S.; Zhou, H.; Chen, X.; Jia, Y.; Zhang, H.; Li, X.; Jiang, Y. 2022: DNMT3A-mediated high expression of circ_0057504 promotes benzo[a]pyrene-induced DNA damage via the NONO-SFPQ complex in human bronchial epithelial cells Environment International 170: 107627
Zeng, Z.; Liu, H.; Yuan, J.; Ren, X.; Deng, Y.; Dai, W.; Wu, Y.; Huang, Y.; Huang, R.; Liu, J.; Huang, H.; Hu, J.'a. 2018: Poly (ADP-ribose) glycohydrolase silencing-mediated maintenance of H2A and downregulation of H2AK9me protect human bronchial epithelial cells from benzo(a)pyrene-induced carcinogenesis Toxicology Letters 295: 270-276
Tischler, A.N.; Levine, G.A.; Bartley, J.C. 1991: Metabolism of benzo[a]pyrene and benzo[a]pyrene-7,8-dihydrodiol in human mammary epithelial cells: feedback inhibition by 7-hydroxybenzo[a]pyrene Carcinogenesis 12(9): 1539-1543
Huang, Y.; Bove, B.; Russo, I.H.; Yang, X.; Russo, J. 1998: Microsatellite instability is induced by benzo pyrene and 7,12-dimethyl benz anthracene in human breast epithelial cells in vitro Proceedings of the American Association for Cancer Research 39: 537
Markovits, P.; Lévy, S.; Nocentini, A.; Velizarov, A.; Sabharwal, P.S.; Benda, P. 1976: In vitro malignant transformation of fetal hamster brain cells by benzo (a) pyrene Comptes Rendus Hebdomadaires des Seances de l'Academie des Sciences. Serie D: Sciences Naturelles 282(22): 2015-2020