+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

In vivo and in vitro evidence for epidermal H2O2-mediated oxidative stress in piebaldism

In vivo and in vitro evidence for epidermal H2O2-mediated oxidative stress in piebaldism

Experimental Dermatology 19(10): 883-887

Piebaldism is characterised by the absence of pigment in patches on the skin, usually present at birth. Mutations in the kit gene are documented. Clinically this disorder can mimic vitiligo. Here, we show for the first time the presence of oxidised pteridine-induced fluorescence in association with H2O2-mediated stress in piebald patches employing Wood's light and in vivo FT-Raman spectroscopy. In situ immunofluorescence data revealed low catalase and methionine sulphoxide reductase A (MSRA) levels whereas thioredoxin reductase and methionine sulphoxide reductase B (MSRB) are not affected. We also show low superoxide dismutase levels in these patients. The presence of thioredoxin reductase provides capacity to reduce H2O2, a mechanism which is absent in vitiligo. Importantly, this enzyme reduces biopterin back to the functioning cofactor 6-tetrahydrobiopterin. The absence of MSRA indicates deficient methionine sulphoxide repair in the cytosol, meanwhile the presence of MSRB is helpful to protect the nucleus. Taken together, we have identified H2O2-mediated stress in piebald skin with distinct differences to vitiligo.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 053778200

Download citation: RISBibTeXText

PMID: 19758321

DOI: 10.1111/j.1600-0625.2009.00966.x

Related references

More evidence for epidermal oxidative stress by H2O2 in vitiligo. Pigment Cell Research 15(Suppl. 9): 32, 2002

Basic evidence for epidermal H2O2/ONOO(-)-mediated oxidation/nitration in segmental vitiligo is supported by repigmentation of skin and eyelashes after reduction of epidermal H2O2 with topical NB-UVB-activated pseudocatalase PC-KUS. Faseb Journal 27(8): 3113-3122, 2013

Influence of salicylic acid on H2O2 production, oxidative stress, and H2O2-metabolizing enzymes. Salicylic acid-mediated oxidative damage requires H2O2. Plant Physiology 115(1): 137-149, 1997

Studies on Hg(II)-induced H2O2 formation and oxidative stress in vivo and in vitro in rat kidney mitochondria. Biochemical Pharmacology 45(10): 2017-2024, 1993

Amyloid beta-mediated oxidative and metabolic stress in rat cortical neurons: no direct evidence for a role for H2O2 generation. Journal of Neurochemistry 67(4): 1595-1606, 1996

In vivo protection of synaptosomes from oxidative stress mediated by Fe2+/H2O2 or 2,2-azobis-(2-amidinopropane) dihydrochloride by the glutathione mimetic tricyclodecan-9-yl-xanthogenate. Free Radical Biology and Medicine 38(8): 1023-1031, 2005

Free Radical Scavenging Properties of Skin and Pulp Extracts of Different Grape Cultivars In Vitro and Attenuation of H2O2-Induced Oxidative Stress in Liver Tissue Ex Vivo. Indian Journal of Clinical Biochemistry 30(3): 305-312, 2015

H2O2 mediates oxidative stress-induced epidermal growth factor receptor phosphorylation. Toxicology Letters (Shannon) 122(3): 205-214, July 6th, 2001

Role of glutaredoxin in metabolic oxidative stress. Glutaredoxin as a sensor of oxidative stress mediated by H2O2. Journal of Biological Chemistry 277(48): 46566-46575, 2002

Fumonisins: oxidative stress-mediated toxicity and metabolism in vivo and in vitro. Archives of Toxicology 90(1): 81, 2016

Nitrofurantoin-induced pulmonary toxicity. In vivo evidence for oxidative stress-mediated mechanisms. Biochemical Pharmacology 43(5): 1127-1135, 1992

In vivo evidence for H2O2 Oxidation of epidermal albumin in vitiligo. Journal of Investigative Dermatology 121(1): 0570, 2003

EUK-134 reduces oxidative stress-mediated renal dysfunction and injury in the rat in vivo and in vitro. Journal of the American Society of Nephrology 12(Program and Abstract Issue): 777A, 2001