+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Inactivation and mutagenesis by phototoxins usingEscherichia coli strains differing in sensitivity to near- and far-ultraviolet light



Inactivation and mutagenesis by phototoxins usingEscherichia coli strains differing in sensitivity to near- and far-ultraviolet light



Journal of Chemical Ecology 12(4): 933-948



FourEscherichia coli strains carrying all the possible combinations of genes controlling sensitivity to near-UV (NUV;nur versusnur (+)) and far-UV (FUV;uvrA6 versusuvrA (+)) were inactivated with broad-spectrum NUV together with specific phototoxins. The inactivation kinetics of the four strains are consistent with the previous reports that psoralen and angelicin inactivation is based on the formation of DNA adducts, while xanthotoxin (8-MOP) inactivation is based on the combined effects of DNA adduct formation and oxygen-dependent photodynamic action. At sufficiently high NUV fluences, xanthotoxol (8-HOP) induces lethal DNA lesions in an excision-deficient (uvrA6) strain. Inactivation by alpha-terthienyl plus NUV involves strictly membrane damage since the genes controlling the sensitivity to either NUV or FUV have no effect on inactivation kinetics. Using mutation to histidine independence (his-4 (+)) in the presence of NUV as a measure of mutagenicity by phototoxins, psoralen and xanthotoxin are mutagenic, angelicin is less mutagenic, and xanthotoxol and alpha-terthienyl are not mutagenic. None of the phototoxins tested in the presence of NUV were as mutagenic as FUV. Imperatorin and berberine were neither phototoxic nor mutagenic in this assay system. This assay thus provides a rapid qualitative screening procedure to identify the mode of action and mutagenicity of plant phototoxins with potential insecticidal properties.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053785734

Download citation: RISBibTeXText

PMID: 24306980

DOI: 10.1007/bf01020262


Related references

Inactivation and mutagenesis by phototoxins using escherichia coli strains differing in sensitivity to near uv and far uv light. Genetics 110(3 Part 2): S20, 1985

Mutation induction by 365-nm radiation and far-ultraviolet light in Escherichia coli strains differing in near- and far-ultraviolet light sensitivity. Mutation Research 126(1): 1-8, 1984

Photodynamic and sun light inactivation of escherichia coli strains differing in near uv sensitivity and recombination proficiency. Photochemistry & Photobiology 31(3): 287-290, 1980

Comparative mutagenesis and interaction between near-ultraviolet (313- to 405-nm) and far-ultraviolet (254-nm) radiation in Escherichia coli strains with differing repair capabilities. Journal of Bacteriology 147(2): 410-417, 1981

Inactivation of Escherichia coli by near-ultraviolet light and 8-methoxypsoralen: different responses of strains B/r and K-12. Journal of Bacteriology 139(2): 454-459, 1979

Comparative mutagenesis of Escherichia coli strains with different repair deficiencies irradiated with 222-nm and 254-nm ultraviolet light. Mutation Research 673(2): 83-86, 2009

Mutagenic DNA repair in Escherichia coli. XVI. Mutagenesis by ultraviolet light plus delayed photoreversal in recA strains. Mutation Research 198(2): 343-350, 1988

Effect of gyrB-mediated changes in chromosome structure on killing of Escherichia coli by ultraviolet light: experiments with strains differing in deoxyribonucleic acid repair capacity. Journal of Bacteriology 146(1): 18-23, 1981

Mutation-proof and mutation-prone modes of survival in derivatives of Escherichia coli differing in sensitivity to ultraviolet light. Brookhaven Symp Biol 20: 17-55, 1967

Ultraviolet light sensitivity of Escherichia coli K-12 strains carrying ruv mutations in combination with uvrA or lon mutant alleles. Journal of Bacteriology 121(2): 735-736, 1975

Differential corneal sensitivity to ultraviolet light among inbred strains of mice. Correlation of ultraviolet B sensitivity with aldehyde dehydrogenase deficiency. Cornea 13(1): 67-72, 1994

Mutagenesis in Escherichia coli. II. Evidence for a common pathway for mutagenesis by ultraviolet light, ionizing radiation and thymine deprivation. Molecular and General Genetics 103(3): 266-273, 1968

Multiple, small exposures of far-ultraviolet or mid-ultraviolet light change the sensitivity to acute ultraviolet exposures measured by cell lethality and mutagenesis in V79 Chinese hamster cells. Radiation Research 114(2): 248-267, 1988

Ultraviolet inactivation of Escherichia coli ribosomes. II. Effects of messenger RNA binding on ultraviolet sensitivity of robosmoes. Biochimica et Biophysica Acta 169(2): 363-372, 1968

Ultraviolet action spectra for aerobic and anaerobic inactivation of Escherichia coli strains specifically sensitive and resistant to near ultraviolet radiations. Photochemistry and Photobiology 38(5): 541-543, 1983