+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Insertion of the Mu1 transposable element into the first intron of maize Adh1 interferes with transcript elongation but does not disrupt chromatin structure



Insertion of the Mu1 transposable element into the first intron of maize Adh1 interferes with transcript elongation but does not disrupt chromatin structure



Plant Molecular Biology 6(6): 441-454



The presence of the Mu1 transposable element within the first intervening sequence of the maize Adh1 gene interfered with transcription through that gene. Insertion of the element did not have an apparent effect on transcription initiation or chromatin structure. In nuclei isolated from anaerobically induced roots, in which Adh1 is transcriptionally active, a subset of the Adh1 chromatin is arranged in a unique conformation characterized by a generalized sensitivity to nucleases, specific DNAase I sensitive sites and a nucleosome array distinct from the inactive configuration present in leaf nuclei. The chromatin organization of the Mu1-induced mutant alleles is indistinguishable from that of the progenitor Adh1-S allele and a point mutant allele that is null for ADH1 activity. The initiation of transcription also proved to be unaffected in these mutants. Nuclear runoff experiments indicated that Adh1 sequences upstream from the point of Mu1 insertion were transcribed normally, but sequences downstream to the insertion were drastically reduced relative to a reference gene expressed in anaerobic root nuclei. Thus, it was concluded that the defect in these Mu1-induced mutants does not reside at the level of gene accessibility or transcript initiation. Rather, Mu1 presents an impediment to the progress of the polymerase II complex during transcript elongation.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053881728

Download citation: RISBibTeXText

PMID: 24307421

DOI: 10.1007/bf00027136


Related references

DNA insertion in the first intron of maize Adh1 affects message levels: cloning of progenitor and mutant Adh1 alleles. Proceedings of the National Academy of Sciences of the United States of America 81(13): 4125-4128, 1984

The Ds1 transposable element acts as an intron in the mutant allele Adh1-Fm335 and is spliced from the message. Nucleic Acids Research 16(9): 3815-3828, 1988

The Dsl transposable element acts as an intron in the mutant allele Adh1-Fm335 and is spliced from the message. Nucleic Acids Research 16(9): 3815-3828, 1988

Chromatin structure at the 44D larval cuticle gene locus in Drosophila: the effect of a transposable element insertion. Nucleic Acids Research 12(23): 9025-9038, 1984

Insertion of Mu1 Elements in the First Intron of the Adh1-S Gene of Maize Results in Novel Rna Processing Events. The Plant Cell 2(12): 1225-1238, 1990

Insertion of Mu1 elements in the first intron of the Adh1-S gene of maize results in novel RNA processing events. Plant Cell 2(12): 1225-1238, 1990

A deletion adjacent to the maize transposable element Mu-1 accompanies loss of Adh1 expression. Embo Journal 4(4): 869-876, 1985

The Mu1 maize transposable element induces tissue-specific aberrant splicing and polyadenylation in two Adh1 mutants. Molecular and Cellular Biology 10(5): 2090-2095, 1990

Dna methylation of the maize transposable element ac interferes with its transcription. Molecular & General Genetics 214(2): 325-327, 1988

Insertion of non-intron sequence into maize introns interferes with splicing. Nucleic Acids Research 20(19): 5181-5187, 1992

De novo synthesis of an intron by the maize transposable element. Journal of Cellular Biochemistry Suppl. (15 Part A): 115, 1991

Insertion of an unstable element in an intervening sequence of maize Adh1 affects transcription but not processing. Proceedings of the National Academy of Sciences of the United States of America 82(9): 2875-2879, 1985

Aberrant transcription caused by the insertion of an early transposable element in an intron of the Fas antigen gene of lpr mice. Proceedings of the National Academy of Sciences of the United States of America 90(5): 1756-1760, 1993

De novo synthesis of an intron by maize transposable element Dissociation. Proceedings of the National Academy of Sciences of the United States of America 91(25): 12150-12154, 1994

De novo synthesis of an intron by the maize transposable element Dissociation. Proceedings of the National Academy of Sciences of the United States of America 91(25): 12150-4, 1994