+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Investigation of polar stationary phases for the separation of sympathomimetic drugs with nano-liquid chromatography in hydrophilic interaction liquid chromatography mode



Investigation of polar stationary phases for the separation of sympathomimetic drugs with nano-liquid chromatography in hydrophilic interaction liquid chromatography mode



Analytica Chimica Acta 685(1): 103-110



In this study, the retention and selectivity of a mixture of basic polar drugs were investigated in hydrophilic interaction chromatographic conditions (HILIC) using nano-liquid chromatography (nano-LC). Six sympathomimetic drugs including ephedrine, norephedrine, synephrine, epinephrine, norepinephrine and norphenylephrine were separated by changing experimental parameters such as stationary phase, acetonitrile (ACN) content, buffer pH and concentration, column temperature. Four polar stationary phases (i.e. cyano-, diol-, aminopropyl-silica and Luna HILIC, a cross-linked diol phase) were selected and packed into fused silica capillary columns of 100 μm internal diameter (i.d.). Among the four stationary phases investigated a complete separation of the all studied compounds was achieved with aminopropyl silica and Luna HILIC stationary phases only. Best chromatographic results were obtained employing a mobile phase composed by ACN/water (92/8, v/v) containing 10mM ammonium formate buffer pH 3. The influence of the capillary temperature on the resolution of the polar basic drugs was investigated in the range between 10 and 50°C. Linear correlation of lnk vs. 1/T was observed for all the columns; ΔH° values were negative with Luna HILIC and positive with aminopropyl- and diol-silica stationary phases, demonstrating that different mechanisms were involved in the separation. To compare the chromatographic performance of the different columns, Van Deemter curves were also investigated.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 053968960

Download citation: RISBibTeXText

PMID: 21168557

DOI: 10.1016/j.aca.2010.11.017


Related references

Separation properties of novel and commercial polar stationary phases in hydrophilic interaction and reversed-phase liquid chromatography mode. Journal of Separation Science 31(9): 1492-1503, 2008

Characterization of polyaniline-coated stationary phases by using the linear solvation energy relationship in the hydrophilic interaction liquid chromatography mode using capillary liquid chromatography. Journal of Separation Science 40(3): 677-687, 2017

Atom-transfer radical graft polymerization initiated directly from silica applied to functionalization of stationary phases for high-performance liquid chromatography in the hydrophilic interaction chromatography mode. Analytical Chemistry 78(20): 7098-7103, 2006

Polar silica-based stationary phases. Part II- Neutral silica stationary phases with surface bound maltose and sorbitol for hydrophilic interaction liquid chromatography. Journal of Chromatography. a 1508: 24-32, 2017

Combination of off-line two-dimensional hydrophilic interaction liquid chromatography for polar fraction and two-dimensional hydrophilic interaction liquid chromatography×reversed-phase liquid chromatography for medium-polar fraction in a traditional Chinese medicine. Journal of Chromatography. a 1224: 61-69, 2012

Stationary phases for hydrophilic interaction liquid chromatography and their applications in separation of traditional Chinese medicines. Se Pu 27(5): 675-681, 2009

Study of the retention behavior of small polar molecules on different types of stationary phases used in hydrophilic interaction liquid chromatography. Journal of Separation Science 37(11): 1297-1307, 2014

The retention behaviour of polar compounds on zirconia based stationary phases under hydrophilic interaction liquid chromatography conditions. Journal of Chromatography. a 1218(39): 6981-6986, 2011

Combination of two different stationary phases for on-line pre-concentration and separation of basic drugs by using nano-liquid chromatography. Journal of Chromatography. a 1285: 118-123, 2013

Combination of off-line two-dimensional hydrophilic interaction liquid chromatography for polar fraction and two-dimensional hydrophilic interaction liquid chromatography ×reversed-phase liquid chromatography for medium-polar fraction in a traditional Chi. 2011

Monolithic poly (SPE-co-BVPE) capillary columns as a novel hydrophilic interaction liquid chromatography stationary phase for the separation of polar analytes. Talanta 100: 344-348, 2012

Characterization and use of hydrophilic interaction liquid chromatography type stationary phases in supercritical fluid chromatography. Journal of Chromatography. a 1250: 182-195, 2012

Novel Stationary Phases for Ion Chromatography and Hydrophilic Interaction Liquid Chromatography. Journal of Analytical Chemistry 74(1): 12-20, 2019

Study of Separation and Identification of the Active Ingredients in Gardenia jasminoides Ellis Based on a Two-Dimensional Liquid Chromatography by Coupling Reversed Phase Liquid Chromatography and Hydrophilic Interaction Liquid Chromatography. Journal of Chromatographic Science 55(1): 75-81, 2017

Preparation of xylitol and maltitol modified silica as novel stationary phases for hydrophilic interaction liquid chromatography and evaluation of their separation performance. Se Pu 33(9): 910-916, 2015