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Investigation of secondary structures and macromolecular interactions in bacteriophage p22 by laser Raman spectroscopy

Investigation of secondary structures and macromolecular interactions in bacteriophage p22 by laser Raman spectroscopy

Biophysical Journal 32(1): 234-237

ISSN/ISBN: 0006-3495

PMID: 19431367

DOI: 10.1016/s0006-3495(80)84945-9

Laser Raman spectra of the DNA bacteriophage P22 and of its precursor particles and related structures have been obtained using 514.5-nm excitation. The spectra show that P22 DNA exists in the B form both inside of the phage head and after extraction from the phage. The major coat protein (gp5) contains a secondary structure composed of 18% alpha-helix, 20% beta-sheet and 62% irregular conformations. The scaffolding protein (gp8) in the phage prohead is substantially richer than gp5 in alpha-helical content. Among the amino acid residues which give prominent Raman lines, the spectra show that tryptophans are exposed to solvent and most tyrosines are hydrogen bonded to positive donor groups. The above features of phage DNA and protein structures are nearly invariant to changes in temperature up to 80 degrees C, indicating a remarkable thermal stability of the phage head and its encapsulated DNA.

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Accession: 053969300

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