+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Investigation on the hosts with natural Paragonimus infection and species identification in Jinhua Prefecture of Zhejiang Province

Investigation on the hosts with natural Paragonimus infection and species identification in Jinhua Prefecture of Zhejiang Province

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 29(5): 348-352

To investigate the natural hosts infected with Paragonimus sp. and identify the species of the parasite in selected counties/districts of Jinhua prefecture in Zhejiang Province. Three townships/towns were randomly sampled from each of the 9 counties/districts in Jinhua as pilot spots for the survey. Fresh-water snails were collected from the fields for examining cercariae. Crabs were collected and detected for metacercariae by routine technique and the metacercariae were fed to dogs purchased in areas free from paragonimiasis. Fecal materials of dogs and cats around the villages and streams where crabs were found infected were collected for examining eggs. The artificially infected dogs were sacrificed 55 d after infection to receive adult worms. The size of cercariae, metacercariae, eggs and adult worms was measured. After the DNA of the adult worm was extracted, PCR was used to amplify the COI gene and ITS2 gene of the mitochondria from the worms. Homology with relative strains/isolates was analyzed and phylogenetic tree constructed. The survey demonstrated that the snail Semisulcospira libertina and the crab Sinopotamon chekiangense served as the first and second intermediate hosts respectively. Natural infection was found in Wucheng District with an infection rate of 0.2% (2/1,088) in snails and 76.7% (46/60) in crabs in Shafan township, and an infection index (II) of 2.0 in crabs, 0.1% (1/1,683) in snails and 53.0% (46/60) in crabs with an II of 0.9 in Langya town. The infection rate was 0 (0/575) in snails and 30.0% (18/60) in crabs with an II of 0.1 in Baimu township of Wuyi County. Paragonimus eggs were detected in feces of stray cats with a positive rate of 8.3% (1/12) in Shafan and 0.6% (1/17) in Langya. The size and morphology of the cercariae, metacercariae, eggs and adult worms were similar to those of Paragonimus westermani. The sequences of the COI and ITS2 genes were with 390 bp and 363 bp respectively, indicating a homology of 88.2%-98.2% and 86.5%-88.1% to the 11 strains/isolates of P. westermani recorded in the GenBank. The phylogenetic trees revealed that the parasite in Jinhua was in between the Minchin (Minqing, Fujian) strain and the Japanese Mie and Chiba strains. Natural paragonimus infection has been detected in snails, The parasite has been identified as P. westermani which is phylogenetically close to those crabs and cats in Jinhua. reported from Fujian Province and from Mie and Chiba of Japan.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 053971850

Download citation: RISBibTeXText

PMID: 24830196

Related references

Epidemiological investigation on the intermediate hosts of Paragonimus in Ninghai County of Zhejiang Province. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 27(4): 373-374, 2009

Fauna investigation on butterfly species in Jinhua, Zhejiang Province. Sichuan Journal of Zoology ; 21(2): 81-83, 2002

New discoveries of Early Cretacous fish fossils in the Jinhua Prefecture, Zhejiang Province. Vertebrata PalAsiatica = Ku Chi Ch'un Tung-wu Yu Ku Jen-lei 14(3): 154-159, 1976

A survey for reservoir hosts of Paragonimus in Zhejiang Province, China. Chinese Journal of Veterinary Medicine Zhongguo Shouyi Zazhi 8(11): 2-6, 1982

First investigation of tick borne Lyme disease in Jinhua, Zhejiang province. Jishengchong Yu Yixue Kunchong Xuebao 14(3): 165-168, 2007

Investigation on the tachinid flies parasitizing the rice insects in Jinhua region, Zhejiang Province. Natural Enemies of Insects 51: 44-48, 1983

First intermediate hosts of Paragonimus spp. in Vietnam and identification of intramolluscan stages of different Paragonimus species. Parasites and Vectors 11(1): 328, 2018

On the distribution of the lung flukes paragonimus africanus and paragonimus uterobilateralis in the southwest province of cameroon and in eastern nigeria as determined by examination of the intermediate crab hosts for infection with metacercariae. Tropenmedizin und Parasitologie 28(1): 120-133, 1977

On saxicolous moss species and their relationships with environmental factors based on ordination analysis in Beishan,Jinhua,Zhejiang Province. Wuhan Zhiwuxue Yanjiu 24(6): 525-530, 2006

Investigation into the ecology of intermediate hosts of Paragonimus skrjabini in northwest Hubei Province. China Tropical Medicine 5(7): 1411-1412, 1433, 2005

The molecular epidemiologic investigation of hantavirus carried by rodent hosts in Wenzhou, Zhejiang province. Zhonghua Liu Xing Bing Xue Za Zhi 29(9): 891-894, 2008

On the natural infection of Paragonimus spp. in cats in Hyogo Prefecture. Japanese Journal of Parasitology 32(4): 333-339, 1983

About the ticks parasitizing on domestic and wild animals in Jinhua, Zhejiang province. Zhongguo Meijieshengwuxue ji Kongzhi Zazhi = Chinese Journal of Vector Biology and Control 18(1): 32-34, 2007

Drought and waterlogging characteristics in Jinhua-Quzhou Basin of Zhejiang Province. Mountain Research: Pages 189-192. 1988., 1988

Natural infection of rodent hosts of paragonimus iloktsuenensis in korea. Korean Journal of Parasitology 9(3 Suppl.): 21-22, 1971