+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Involvement of mitochondrial pathway in NCTD-induced cytotoxicity in human hepG2 cells

Involvement of mitochondrial pathway in NCTD-induced cytotoxicity in human hepG2 cells

Journal of Experimental and Clinical Cancer Research 29: 145

Norcantharidin, the demethylated analog of cantharidin derived from a traditional Chinese medicine, Mylabris, has been used in the treatment of anti-cancer effects. However, the detailed mechanisms underlying this process are generally unclear. The aim of this study was to investigate the mechanism of NCTD-induced apoptosis in HepG2 cells. The cytotoxicity was measured by MTT assay for cellular viability and by flow cytometry. The mitochondrial membrane potential and reactive oxygen species production was evaluated by flow cytometry analysis. The role of caspase activities were assayed using caspase apoptosis detection kit . Western blot analysis was used to evaluate the level of Cyto-C, Bcl-2, Bax, Bid, caspase 3, -9, -8 and PARP expression After treatment with NCTD, a decrease in the viability of HepG2 cells and increase in apoptosis were observed. NCTD-induced apoptosis was accompanied by an increase in ROS production, loss of mitochondrial membrane potential and release of cytochrome c(cyto-c) from the mitochondria to the cytosol and down-regulation of anti-apoptotic protein Bcl-2 levels with concurrent up-regulation in pro-apoptotic protein Bax levels. However, another pro-apoptotic molecule, Bid, showed no change in such same treatment. NCTD-increased activity of caspase 9,caspase 3 and the subsequent cleavage caspase substrate PARP were also observed. The expression levels of pro-caspase-8 were not changed after NCTD treatment. These results indicate that NCTD induced cytotoxicity in HepG2 cells by apoptosis, which is mediated through ROS generation and mitochondrial pathway.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 053977587

Download citation: RISBibTeXText

PMID: 21059274

DOI: 10.1186/1756-9966-29-145

Related references

Involvement of mitochondrial pathway in triptolide-induced cytotoxicity in human normal liver L-02 cells. Biological & Pharmaceutical Bulletin 31(4): 592-597, 2008

Embelin-induced apoptosis of HepG2 human hepatocellular carcinoma cells and blockade of HepG2 cells in the G2/M phase via the mitochondrial pathway. Experimental and Therapeutic Medicine 4(4): 649-654, 2012

Oligomeric proanthocyanidins alleviate hexabromocyclododecane-induced cytotoxicity in HepG2 cells through regulation on ROS formation and mitochondrial pathway. Toxicology in Vitro 28(2): 319-326, 2014

Involvement of enniatins-induced cytotoxicity in human HepG2 cells. Toxicology Letters 218(2): 166-173, 2013

Possible involvement of glutathione balance disruption in dihydropyrazine-induced cytotoxicity on human hepatoma HepG2 cells. Journal of Toxicological Sciences 37(5): 1065-1069, 2012

Possible involvement of glutathione balance disruption in dihydropyrazine-induced cytotoxicity on human hepatoma HepG2 cells. The Journal of Toxicological Sciences 37(5): 1065-1069, 2012

Streptozotocin-induced cytotoxicity, oxidative stress and mitochondrial dysfunction in human hepatoma HepG2 cells. International Journal of Molecular Sciences 13(5): 5751-5767, 2012

Oxidative stress and mitochondrial injury-mediated cytotoxicity induced by silver nanoparticles in human A549 and HepG2 cells. Environmental Toxicology 31(12): 1691-1699, 2016

Apoptosis in HepG2 cells induced by zinc pyrithione via mitochondrial dysfunction pathway: Involvement of zinc accumulation and oxidative stress. Ecotoxicology and Environmental Safety 161: 515-525, 2018

MAC-related mitochondrial pathway in oroxylin-A-induced apoptosis in human hepatocellular carcinoma HepG2 cells. Cancer Letters 284(2): 198-207, 2009

Biguanide-induced mitochondrial dysfunction yields increased lactate production and cytotoxicity of aerobically-poised HepG2 cells and human hepatocytes in vitro. Toxicology and Applied Pharmacology 233(2): 203-210, 2008

Fucoxanthin Enhances Cisplatin-Induced Cytotoxicity via NFamp;amp;#954;B-Mediated Pathway and Downregulates DNA Repair Gene Expression in Human Hepatoma HepG2 Cells. 2013

Fucoxanthin enhances cisplatin-induced cytotoxicity via NFκB-mediated pathway and downregulates DNA repair gene expression in human hepatoma HepG2 cells. Marine Drugs 11(1): 50-66, 2013

Involvement of intrinsic mitochondrial pathway in neosergeolide-induced apoptosis of human HL-60 leukemia cells: the role of mitochondrial permeability transition pore and DNA damage. Pharmaceutical Biology 50(8): 980-993, 2012

Involvement of mitochondrial pathway in Taxol-induced apoptosis of human T24 bladder cancer cells. Urological Research 30(5): 282-288, 2002