+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Characterization of AHR2 and CYP1A expression in Atlantic sturgeon and shortnose sturgeon treated with coplanar PCBs and TCDD

Characterization of AHR2 and CYP1A expression in Atlantic sturgeon and shortnose sturgeon treated with coplanar PCBs and TCDD

Aquatic Toxicology 197: 19-31

ISSN/ISBN: 0166-445X

PMID: 29427830

DOI: 10.1016/j.aquatox.2018.01.017

Atlantic sturgeon and shortnose sturgeon co-occur in many estuaries along the Atlantic Coast of North America. Both species are protected under the U.S. Endangered Species Act and internationally on the IUCN Red list and by CITES. Early life-stages of both sturgeons may be exposed to persistent aromatic hydrocarbon contaminants such as PCBs and PCDD/Fs which are at high levels in the sediments of impacted spawning rivers. Our objective was to compare the PCBs and TCDD sensitivities of both species with those of other fishes and to determine if environmental concentrations of these contaminants approach those that induce toxicity to their young life-stages under controlled laboratory conditions. Because our previous studies suggested that young life-stages of North American sturgeons are among the more sensitive of fishes to coplanar PCB and TCDD-induced toxicities, we were interested in identifying the molecular bases of this vulnerability. It is known that activation of the aryl hydrocarbon receptor 2 (AHR2) in fishes mediates most toxicities to these contaminants and transcriptional activation of xenobiotic metabolizing enzymes such as cytochrome P4501A (CYP1A). Previous studies demonstrated that structural and functional variations in AHRs are the bases for differing sensitivities of several vertebrate taxa to aromatic hydrocarbons. Therefore, in this study we characterized AHR2 and its expression in both sturgeons as an initial step in understanding the mechanistic bases of their sensitivities to these contaminants. We also used CYP1A expression as an endpoint to develop Toxicity Equivalency Factors (TEFs) for these sturgeons. We found that critical amino acid residues in the ligand binding domain of AHR2 in both sturgeons were identical to those of the aromatic hydrocarbon-sensitive white sturgeon, and differed from the less sensitive lake sturgeon. AHR2 expression was induced by TCDD (up to 6-fold) and by three of four tested coplanar PCB congeners (3-5-fold) in Atlantic sturgeon, but less so in shortnose sturgeon. We found that expression of AHR2 and CYP1A mRNA significantly covaried after exposure to TCDD and PCB77, PCB81, PCB126, but not PCB169 in both sturgeons. We also determined TEFs for the four coplanar PCBs in shortnose sturgeon based on comparison of CYP1A mRNA expression across all doses. Surprisingly, the TEFs for all four coplanar PCBs in shortnose sturgeon were much higher (6.4-162 times) than previously adopted for fishes by the WHO.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 054006914

Download citation: RISBibTeXText

Related references

Characterization of AHR2 and CYP1A expression in Atlantic sturgeon and shortnose sturgeon treated with coplanar PCBs and TCDD. Aquatic Toxicology 197: 19-31, 2018

Characterization and expression of cytochrome P4501A in Atlantic sturgeon and shortnose sturgeon experimentally exposed to coplanar PCB 126 and TCDD. Aquatic Toxicology 104(1-2): 23-31, 2011

Toxic effects of PCB126 and TCDD on shortnose sturgeon and Atlantic sturgeon. Environmental Toxicology and Chemistry 31(10): 2324-2337, 2012

Characterization of AHR1 and its functional activity in Atlantic sturgeon and shortnose sturgeon. Aquatic Toxicology 205: 25-35, 2018

Impacts of life history and biogeography on the genetic stock structure of Atlantic sturgeon Acipenser oxyrinchus oxyrinchus, Gulf sturgeon A. oxyrinchus desotoi, and shortnose sturgeon A. brevirostrum. Journal of Applied Ichthyology 18(4/6): 509-518, 2002

Habitats used by shortnose sturgeon in two Massachusetts rivers, with notes on estuarine Atlantic sturgeon: A hierarchical approach. Transactions of the American Fisheries Society 129(2): 487-503, 2000

Seasonal Distribution and Movements of Shortnose Sturgeon and Atlantic Sturgeon in the Penobscot River Estuary, Maine. Transactions of the American Fisheries Society 139(5): 1436-1449, 2010

Evaluation of tagging techniques for shortnose sturgeon and Atlantic sturgeon. American Fisheries Society Symposium 7: 134-141, 1990

Experimental Examination of Surgical Procedures for Implanting Sonic Transmitters in Juvenile Shortnose Sturgeon and Atlantic Sturgeon. North American Journal of Fisheries Management 33(3): 549-556, 2013

Shortnose Sturgeon and Atlantic Sturgeon in the Kennebec River System, Maine: a 19772001 Retrospective of Abundance and Important Habitat. Transactions of the American Fisheries Society 144(3): 591-601, 2015

Effect of feeding frequency on growth, food conversion efficiency, and meal size of juvenile Atlantic sturgeon and shortnose sturgeon. North American Journal of Aquaculture il; 65(2): 99-105, 2003

Hepatic Burdens of PCB and PCDD/F Congeners in Federally Endangered Shortnose Sturgeon and Atlantic Sturgeon from the Hudson River, new York, USA: Burden Patterns and Potential Consequences in Offspring. Archives of Environmental Contamination and Toxicology 83(1): 21-35, 2022

Effects of temperature on the early development, growth, and survival of shortnose sturgeon, Acipenser brevirostrum, and Atlantic sturgeon, Acipenser oxyrhynchus, yolk-sac larvae. Environmental Biology of Fishes e; 70(2): 145-154, 2004

Ontogenetic behavior and migration of Atlantic sturgeon, Acipenser oxyrinchus oxyrinchus, and shortnose sturgeon, A. brevirostrum, with notes on social behavior. Environmental Biology of Fishes 63(2): 137-150, 2002

Use of laparoscopy to determine sex and reproductive status of shortnose sturgeon Acipenser brevirostrum and Atlantic sturgeon Acipenser oxyrinchus oxyrinchus. Journal of Applied Ichthyology 27. 2, 2011