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Isolation and characterization of RAPD-based markers linked to the beet cyst nematode resistance locus (Hs1 (pat-1)) on chromosome 1 of B. patellaris



Isolation and characterization of RAPD-based markers linked to the beet cyst nematode resistance locus (Hs1 (pat-1)) on chromosome 1 of B. patellaris



Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 90(6): 885-891



A beet cyst nematode (BCN)-resistant telosomic addition of B. patellaris chromosome 1 in B. vulgaris was used to isolate 6 RAPD markers linked to the BCN resistance locus Hs1(pat-1). Southern analysis showed that the analyzed RAPD products contain either low-, middle- or high-repetitive DNA. The relative positions of the random amplified polymorphic DNA (RAPD) markers and of the restriction fragment length polymorphism (RFLP) loci corresponding to the low-repetitive RAPD products were determined by deletion mapping using a panel of seven nematode-resistant B. patellaris chromosome-1 fragment additions. One RAPD marker, OPB11(800), was found to be present in two copies on the long arm telosome of B. patellaris chromosome 1. These copies are closely linked to the BCN resistance gene and flank the gene on both sides. On the basis of the nucleotide sequence of OPB11(800), sequence-tagged site (STS) primers were developed that amplify specific fragments derived from the two OPB11(800) loci. These STS markers can be used in the map-based cloning of the BCN gene, as they define start and finishing points of a chromosomal walk towards the Hs1(pat-1) - locus. Two copies of the middle-repetitive OPX2(1100) marker were mapped in the same interval of the deletion mapping panel as the resistance gene locus and thereby belong to the nearest markers as vet found for the BCN gene in B. patellaris.

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Accession: 054011334

Download citation: RISBibTeXText

PMID: 24172934

DOI: 10.1007/bf00222027


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