+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Isolation and identification of pelteobagrin, a novel antimicrobial peptide from the skin mucus of yellow catfish (Pelteobagrus fulvidraco)



Isolation and identification of pelteobagrin, a novel antimicrobial peptide from the skin mucus of yellow catfish (Pelteobagrus fulvidraco)



Comparative Biochemistry and Physiology. Part B, Biochemistry and Molecular Biology 158(2): 149-154



Fish skin mucus has recently been recognized to be a potential source of antimicrobial peptides, which provides the first line of defense against invading pathogens. This study reports the purification and characterization of a novel linear antimicrobial peptide, pelteobagrin, from the skin mucus of yellow catfish (Pelteobagrus fulvidraco, Richardson). Pelteobagrin is 20 amino acids in length (GKLNLFLSRLEILKLFVGAL) and shows no clear homology with any known bioactive peptides. MALDI-TOF MS indicated the molecular mass of the purified peptide was 2244.4 Da, which is in good agreement with pelteobagrin's predicted molecular weight of 2244.8 Da. Pelteobagrin exhibited antibacterial activity against Gram-positive and Gram-negative bacteria as well as fungi, and the activity was relatively salt-insensitive as it was not affected by NaCl concentrations of up to 137 mM. Moreover, pelteobagrin displayed no hemolytic activity to rabbit red blood cells. Transmission electron microscopy suggested that pelteobagrin might kill bacteria via acting on both the cell wall and the cytoplasmic membrane of bacteria. These results suggest that pelteobagrin might be involved in the innate defense system in yellow catfish.

(PDF emailed within 0-6 h: $19.90)

Accession: 054013256

Download citation: RISBibTeXText

PMID: 21073979

DOI: 10.1016/j.cbpb.2010.11.002


Related references

Chemotactic Activity of Cyclophilin A in the Skin Mucus of Yellow Catfish (Pelteobagrus fulvidraco) and Its Active Site for Chemotaxis. International Journal of Molecular Sciences 17(9), 2017

Microbial diversity of intestinal contents and mucus in yellow catfish Pelteobagrus fulvidraco. Aquaculture (May 24): 1-7, 2010

RAPD markers between yellow catfish Pelteobagrus fulvidraco and long yellow catfish Pelteobagrus eupogon. 2006

Isolation and characterization of twelve novel microsatellites in yellow catfish, Pelteobagrus fulvidraco. Conservation Genetics 10(3): 755-757, 2009

Isolation and analysis of Sox9 derived from yellow catfish (Pelteobagrus fulvidraco). Journal of Agricultural Biotechnology 13(5): 620-623, 2005

Isolation and analysis of sox9 gene derived from yellow catfish Pelteobagrus fulvidraco. Chinese Journal of Agricultural Biotechnology 3(2): 109-114, 2006

Isolation and characterization of polymorphic microsatellite loci from yellow catfish Pelteobagrus fulvidraco. Conservation Genetics Resources 1(1): 313-315, 2009

Isolation and characterization of polymorphic microsatellite markers in the yellow catfish, Pelteobagrus fulvidraco. Conservation Genetics Resources 1(1): 63-66, 2009

Isolation and characterization of 57 novel polynucleotide microsatellites from yellow catfish Pelteobagrus fulvidraco genome for genetic analysis. Conservation Genetics Resources 6(1): 73-77, 2014

RAPD (random amplification of polymorphic DNA) markers between yellow catfish (Pelteobagrus fulvidraco) and long whiskers yellow catfish (P. eupogon). 2006

Gonadal transcriptomic analysis of yellow catfish (Pelteobagrus fulvidraco): identification of sex-related genes and genetic markers. Physiological Genomics 46(21): 798-807, 2015

Identification of an isolate of Saprolegnia ferax as the causal agent of saprolegniosis of yellow catfish (Pelteobagrus fulvidraco) eggs. Veterinary Research Communications 36(4): 239-244, 2013

Effects of dietary protein to carbohydrate ratios on growth and body composition of juvenile yellow catfish, Pelteobagrus fulvidraco Siluriformes, Bagridae, Pelteobagrus. Aquaculture Research 40(12): 1410-1418, 2009

Isolation and characterization of Edwardsiella ictaluri strains as pathogens from diseased yellow catfish Pelteobagrus fulvidraco Richardson cultured in China. Aquaculture Research 41(12): 1835-1844, 2010

Molecular identification and expression analysis of a goose-type lysozyme (LysG) gene in yellow catfish Pelteobagrus fulvidraco. Fish and Shellfish Immunology 58: 423-428, 2016