+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Knockdown of leptin A expression dramatically alters zebrafish development



Knockdown of leptin A expression dramatically alters zebrafish development



General and Comparative Endocrinology 178(3): 562-572



Using morpholino antisense oligonucleotide (MO) technology, we blocked leptin A or leptin receptor expression in embryonic zebrafish, and analyzed consequences of leptin A knock-down on fish development. Embryos injected with leptin A or leptin receptor MOs (leptin A or leptin receptor morphants) had smaller bodies and eyes, undeveloped inner ear, enlarged pericardial cavity, curved body and/or tail and larger yolk compared to control embryos of the same stages. The defects persisted in 6-9 days old larvae. We found that blocking leptin A function had little effect on the development of early brain (1 day old), but differentiation of both the morphant dorsal brain and retinal cells was severely disrupted in older (2 days old) embryos. Despite the enlarged pericardial cavity, differentiation of cardiac cells appeared to be similar to control embryos. Formation of the morphants' inner ear is also severely disrupted, which corroborates existing reports of leptin receptor expression in inner ear of both zebrafish and mammals. Co-injection of leptin A MO and recombinant leptin results in partial rescue of the wild-type phenotype. Our results suggest that leptin A plays distinct roles in zebrafish development.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 054048805

Download citation: RISBibTeXText

PMID: 22841760

DOI: 10.1016/j.ygcen.2012.07.011


Related references

The knockdown of maternal glucocorticoid receptor mRNA alters embryo development in zebrafish. Developmental Dynamics 240(4): 874-889, 2011

Deiodinase knockdown affects zebrafish eye development at the level of gene expression, morphology and function. Molecular and Cellular Endocrinology 424: 81-93, 2016

Ectopic expression and knockdown of a zebrafish sox21 reveal its role as a transcriptional repressor in early development. Mechanisms of Development 121(2): 131-142, 2004

Knockdown of zebrafish YY1a can downregulate the phosphatidylserine (PS) receptor expression, leading to induce the abnormal brain and heart development. Journal of Biomedical Science 23: 31, 2016

Knockdown of a zebrafish aryl hydrocarbon receptor repressor (AHRRa) affects expression of genes related to photoreceptor development and hematopoiesis. Toxicological Sciences 139(2): 381-395, 2014

Knockdown of the hypertension-associated gene NOSTRIN alters glomerular barrier function in zebrafish (Danio rerio). Hypertension 62(4): 726-730, 2013

Knockdown of the intraflagellar transport protein IFT46 stimulates selective gene expression in mouse chondrocytes and affects early development in zebrafish. Journal of Biological Chemistry 282(42): 30960-30973, 2007

Helicobacter pylori alters leptin and leptin receptor expression in human gastric epithelial cells. Gut 47(Suppl. 1): A35, 2000

The defective expression of gtpbp3 related to tRNA modification alters the mitochondrial function and development of zebrafish. International Journal of Biochemistry and Cell Biology 77(Pt A): 1-9, 2016

Placental restriction increases adipose leptin gene expression and plasma leptin and alters their relationship to feeding activity in the young lamb. Pediatric Research 67(6): 603-608, 2010

Deiodinase knockdown during early zebrafish development affects growth, development, energy metabolism, motility and phototransduction. Plos one 10(4): E0123285, 2015

Conservation, expression, and knockdown of zebrafish plxnb2a and plxnb2b. Developmental Dynamics 239(10): 2722-2734, 2010

Mutation that dramatically alters rat titin isoform expression and cardiomyocyte passive tension. Journal of Molecular and Cellular Cardiology 44(6): 983-991, 2008

Leptin and leptin receptor expression in the myometrium and uterine myomas: Is leptin involved in tumor development?. International Journal of Oncology 27(6): 1505-1509, 2005

Follicular fluid leptin concentrations and expression of leptin and leptin receptor in the equine ovary and in vitro-matured oocyte with reference to pubertal development and breeds. Reproduction Fertility and Development 25(5): 837-846, 2013