Layer-by-layer buildup of poly (L-glutamic acid) /chitosan film for biologically active coating
Song, Z.; Yin, J.; Luo, K.; Zheng, Y.; Yang, Y.; Li, Q.; Yan, S.; Chen, X.
Macromolecular Bioscience 9(3): 268-278
ISSN/ISBN: 1616-5195 PMID: 18855946 DOI: 10.1002/mabi.200800164
A new biocompatible film based on chitosan and poly(L-glutamic acid) (CS/PGA), created by alternate deposition of CS and PGA, was investigated. FT-IR spectroscopy, UV-vis spectroscopy and QCM were used to analyze the build-up process. The growth of CS and PGA deposition are both exponential to the deposition steps at first. After about 9 (CS/PGA) depositions, the exponential to linear transition takes place. QCM measurements combined with UV-vis spectra revealed the increase in the multilayer film growth at different pH (4.4, 5.0 and 5.5). The build-up of the multilayer stops after a few depositions at pH = 6.5. A muscle myoblast cell (C2C12) assay showed that (CS/PGA)(n) multilayer films obviously promote C2C12 attachment and growth.