+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterizing Intact Macromolecular Complexes Using Native Mass Spectrometry

Characterizing Intact Macromolecular Complexes Using Native Mass Spectrometry

Methods in Molecular Biology 1764: 133-151

Native mass spectrometry (MS) enables the characterization of macromolecular assemblies with high sensitivity. It can reveal the stoichiometry of subunits as well as their two-dimensional interaction network and provide information regarding the dynamic behavior of macromolecular complexes. Here, we describe the workflow to perform native MS experiments. In addition, we illustrate the quality control analysis of proteins using MS in denaturing conditions.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 054110723

Download citation: RISBibTeXText

PMID: 29605913

DOI: 10.1007/978-1-4939-7759-8_9

Related references

The emerging role of native mass spectrometry in characterizing the structure and dynamics of macromolecular complexes. Protein Science 24(8): 1176-1192, 2015

Native mass spectrometry characterization of intact nanodisc lipoprotein complexes. Analytical Chemistry 84(21): 8957-8960, 2012

Characterization of intact protein conjugates and biopharmaceuticals using ion-exchange chromatography with online detection by native electrospray ionization mass spectrometry and top-down tandem mass spectrometry. Analytical Chemistry 87(19): 10138-10145, 2015

Charge variant native mass spectrometry benefits mass precision and dynamic range of monoclonal antibody intact mass analysis. Mabs 10(8): 1214-1225, 2018

Mass spectrometric analysis of intact macromolecular chaperone complexes. Drug Discovery Today. Technologies 3(1): 21-27, 2006

Investigating macromolecular complexes using top-down mass spectrometry. Proteomics 14(10): 1259-1270, 2014

Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs. Chemical Communications 54(97): 13702-13705, 2018

Use of a charge reducing agent to enable intact mass analysis of cysteine-linked antibody-drug-conjugates by native mass spectrometry. Eupa Open Proteomics 11: 23-27, 2016

Investigation of molecular interaction within biological macromolecular complexes by mass spectrometry. Medicinal Research Reviews 26(3): 339-368, 2006

Radiolytic protein footprinting with mass spectrometry to probe the structure of macromolecular complexes. Annual Review of Biophysics and Biomolecular Structure 35: 251-276, 2006

Native Top-Down Mass Spectrometry and Ion Mobility MS for Characterizing the Cobalt and Manganese Metal Binding of α-Synuclein Protein. Journal of the American Society for Mass Spectrometry 29(9): 1870-1880, 2018

Application of Electrospray Mass Spectrometry for Characterizing Supramolecular Coordination Complexes. Helvetica Chimica Acta 76(4): 1759-1766, 1993

Native protein mass spectrometry: from intact oligomers to functional machineries. Current Opinion in Chemical Biology 8(5): 519-526, 2004

Characterizing the lipid and metabolite changes associated with placental function and pregnancy complications using ion mobility spectrometry-mass spectrometry and mass spectrometry imaging. Placenta 60(Suppl. 1): S67-S72, 2017

Single-Step Affinity Purification (ssAP) and Mass Spectrometry of Macromolecular Complexes in the Yeast S. cerevisiae. Methods in Molecular Biology 1361: 265-287, 2016