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Characterizing Intact Macromolecular Complexes Using Native Mass Spectrometry



Characterizing Intact Macromolecular Complexes Using Native Mass Spectrometry



Methods in Molecular Biology 1764: 133-151



Native mass spectrometry (MS) enables the characterization of macromolecular assemblies with high sensitivity. It can reveal the stoichiometry of subunits as well as their two-dimensional interaction network and provide information regarding the dynamic behavior of macromolecular complexes. Here, we describe the workflow to perform native MS experiments. In addition, we illustrate the quality control analysis of proteins using MS in denaturing conditions.

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Accession: 054110723

Download citation: RISBibTeXText

PMID: 29605913

DOI: 10.1007/978-1-4939-7759-8_9


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