+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Low-level dietary deoxynivalenol and acute exercise stress result in immunotoxicity in BALB/c mice



Low-level dietary deoxynivalenol and acute exercise stress result in immunotoxicity in BALB/c mice



Journal of Immunotoxicology 3(4): 173-178



We hypothesized that acute exercise stress would exacerbate immunosuppressive effects of sub-acute exposure to dietary deoxynivalenol (DON). Male BALB/c mice were fed 0 or 2 mg DON/kg diet for 14 days, 12 animals per dose, and then exercised to fatigue on a treadmill. Mice were euthanized by decapitation, trunk blood and spleens were collected. Single-cell suspensions of splenocytes were used to quantify immune function by plaque hemolysis and conconavalin-A (ConA) stimulated lymphocyte proliferation assays. Serum corticosterone level was determined by enzyme immunoassay. Only the nonexercised DON-fed mice showed significant splenocyte proliferation suppression, 32.9 +/- 17.9% of nonexercised controls (p = 0.021). Exercised controls and DON-fed exercised animals showed splenocyte proliferation of 68-75% of nonexercised controls. Antibody response to a T-dependent antigen, sheep red blood cells, was significantly less for exercised DON-fed mice than in controls (p = 0.031). Serum corticosterone levels were significantly higher for both exercised groups compared to the unexercised groups (p < 0.001). IL-4 secretion from mitogen-stimulated splenocytes was elevated by DON alone (p < 0.05) while IL-2 was elevated by DON with exercise stress (p < 0.05). Our hypothesis was confirmed with respect to T-lymphocyte-dependent antibody production, but not for splenocyte proliferation. Exercise stress abrogated DON-mediated suppression of splenocyte proliferation, perhaps mediated by induction of elevated stress hormones counteracting cytokine expression alterations of DON.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 054190160

Download citation: RISBibTeXText

PMID: 18958698

DOI: 10.1080/15476910600979978


Related references

Benzanthrone induced immunotoxicity via oxidative stress and inflammatory mediators in Balb/c mice. Immunobiology 220(3): 369-381, 2015

Does Stress Result in You Exercising Less? Or Does Exercising Result in You Being Less Stressed? Or Is It Both? Testing the Bi-directional Stress-Exercise Association at the Group and Person (N of 1) Level. Annals of Behavioral Medicine 51(6): 799-809, 2017

Synthesis and characterization of deoxynivalenol glucuronide: its comparative immunotoxicity with deoxynivalenol. Food and Chemical Toxicology 45(10): 1846-1855, 2007

Immunotoxicity of atrazine in Balb/c mice. Journal of Environmental Science and Health. Part. B Pesticides Food Contaminants and Agricultural Wastes 48(8): 637-645, 2013

Immunotoxicity of acrylamide in female BALB/c mice. Biomedical and Environmental Sciences 27(6): 401-409, 2014

Type and level of dietary fat does not effect the growth of ct 26 implanted subcutaneously in balb c mice. FASEB Journal 5(5): A1285, 1991

Immunotoxicity testing of mycotoxins T-2 and patulin on Balb/c mice. Acta Microbiologica Hungarica 37(4): 331-339, 1990

Hematotoxicity of deoxynivalenol in BALB/c mice. Journal of Pharmacology and Pharmacotherapeutics 2(2): 115-117, 2011

Immunotoxicity of the organochlorine pesticide methoxychlor in female ICR, BALB/c, and C3H/He mice. Journal of Immunotoxicology 10(2): 119-124, 2013

Zinc oxide nanoparticle induced age dependent immunotoxicity in BALB/c mice. Toxicology Research 6(3): 342-352, 2017

Subchronic inhalation toxicity of isobutyl nitrite in BALB/c mice. II. Immunotoxicity studies. Journal of Toxicology and Environmental Health 15(6): 835-846, 1985

Immunotoxicity assessment of CdSe/ZnS quantum dots in macrophages, lymphocytes and BALB/c mice. Journal of Nanobiotechnology 14: 10, 2016

Patterns of immunotoxicity associated with chronic as compared with acute exposure to chemical or physical stressors and their relevance with regard to the role of stress and with regard to immunotoxicity testing. Toxicological Sciences 109(2): 265-275, 2009

Immunotoxicity of organophosphorous pesticides, methidathion and pirimiphos-methyl on the immune system of Balb/c mice. Toxicology Letters (Shannon) 144(Suppl 1): s39-s40, 2003

Role of glutathione conjugation in the hepatotoxicity and immunotoxicity induced by 1-bromopropane in female BALB/c mice. Journal of Applied Toxicology 27(4): 358-367, 2007