+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Lymph node transplantation results in spontaneous lymphatic reconnection and restoration of lymphatic flow

Lymph node transplantation results in spontaneous lymphatic reconnection and restoration of lymphatic flow

Plastic and Reconstructive Surgery 133(2): 301-310

Although lymph node transplantation has been shown to improve lymphatic function, the mechanisms regulating lymphatic vessel reconnection and functional status of lymph nodes remains poorly understood. The authors developed and used LacZ lymphatic reporter mice to examine the lineage of lymphatic vessels infiltrating transferred lymph nodes. In addition, the authors analyzed lymphatic function, expression of vascular endothelial growth factor (VEGF)-C, maintenance of T- and B-cell zone, and anatomical localization of lymphatics and high endothelial venules. Reporter mice were specific and highly sensitive in identifying lymphatic vessels. Lymph node transfer was associated with rapid return of lymphatic function and clearance of technetium-99 secondary to a massive infiltration of recipient mouse lymphatics and putative connections to donor lymphatics. T- and B-cell populations in the lymph node were maintained. These changes correlated with marked increases in the expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Newly formed lymphatic channels in transferred lymph nodes were in close anatomical proximity to high endothelial venules. Transferred lymph nodes have rapid infiltration of functional host lymphatic vessels and maintain T- and B-cell populations. This process correlates with increased endogenous expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Anatomical proximity of newly formed lymphatics and high endothelial venules supports the hypothesis that lymph node transfer can improve lymphedema by exchanges with the systemic circulation.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 054200103

Download citation: RISBibTeXText

PMID: 24469165

DOI: 10.1097/01.prs.0000436840.69752.7e

Related references

Lymphatic Reconnection and Restoration of Lymphatic Flow by Nonvascularized Lymph Node Transplantation: Real-Time Fluorescence Imaging Using Indocyanine Green and Fluorescein Isothiocyanate-Dextran. Lymphatic Research and Biology 16(2): 165-173, 2018

Lymph Flow Restoration after Tissue Replantation and Transfer: Importance of Lymph Axiality and Possibility of Lymph Flow Reconstruction without Lymph Node Transfer or Lymphatic Anastomosis. Plastic and Reconstructive Surgery 142(3): 796-804, 2018

Successful lymph node transplantation in rats, with restoration of lymphatic function. Plastic and Reconstructive Surgery 63(6): 817-823, 1979

VEGF-C improves regeneration and lymphatic reconnection of transplanted autologous lymph node fragments: An animal model for secondary lymphedema treatment. Immunity Inflammation and Disease 2(3): 152-161, 2014

Restoration of lymphatic vessels following lymph node extirpation. Biulleten' Eksperimental'noi Biologii i Meditsiny 68(8): 109-112, 1969

Intraoperative Detection of Efferent Lymphatic Vessels Emerging from Lymph Node during Lymphatic Microsurgery. Journal of Reconstructive Microsurgery 35(5): 372-378, 2019

The Lymphatic Endothelial mCLCA1 Antibody Induces Proliferation and Growth of Lymph Node Lymphatic Sinuses. Plos one 11(5): E0156079, 2016

Lymphatic vessel function and lymphatic growth factor secretion after microvascular lymph node transfer in lymphedema patients. Plastic and Reconstructive Surgery. Global Open 1(2): 1-9, 2013

Lymphatic Leak Occurring After Surgical Lymph Node Dissection: A Preliminary Study Assessing the Feasibility and Outcome of Lymphatic Embolization. Cardiovascular and Interventional Radiology 39(12): 1728-1735, 2016

Adipose-Derived Stem Cells and Vascularized Lymph Node Transfers Successfully Treat Mouse Hindlimb Secondary Lymphedema by Early Reconnection of the Lymphatic System and Lymphangiogenesis. Plastic and Reconstructive Surgery 139(3): 639-651, 2017

Tumor Regulation of Lymph Node Lymphatic Sinus Growth and Lymph Flow in Mice and in Humans. Yale Journal of Biology and Medicine 90(3): 403-415, 2017

Cutaneous melanoma: therapeutic lymph node and elective lymph node dissections, lymphatic mapping, and sentinel lymph node biopsy. Dermatologic Therapy 18(6): 397-406, 2005

Lymphatic lymphatic interactions can reduce lymph flow and potentiate organ edema formation. FASEB Journal 2(6): ABSTRACT M91, 1988

D2-40 lymphatic marker for detecting lymphatic invasion in thin to intermediate thickness melanomas: association with sentinel lymph node status and prognostic value-a retrospective case study. Journal of the American Academy of Dermatology 64(2): 336-345, 2011

Lymph node transplantation for the study of lymphatic metastasis of tumor. Lymphology 20(1): 48-49, 1987