+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

MLVA16 typing of Portuguese human and animal Brucella melitensis and Brucella abortus isolates



MLVA16 typing of Portuguese human and animal Brucella melitensis and Brucella abortus isolates



Plos One 7(8): E42514



To investigate the epidemiological relationship of isolates from different Portuguese geographical regions and to assess the diversity among isolates, the MLVA16(Orsay) assay (panels 1, 2A and 2B) was performed with a collection of 126 Brucella melitensis (46 human and 80 animal isolates) and 157 B. abortus field isolates, seven vaccine strains and the representative reference strains of each species. The MLVA16(Orsay) showed a similar high discriminatory power (HGDI 0.972 and 0.902) for both species but panel 1 and 2A markers displayed higher diversity (HGDI 0.693) in B. abortus compared to B. melitensis isolates (HGDI 0.342). The B. melitensis population belong to the "Americas" (17%) and "East Mediterranean" (83%) groups. No isolate belonged to the "West Mediterranean" group. Eighty-five percent of the human isolates (39 in 46) fit in the "East-Mediterranean" group where a single lineage known as MLVA11 genotype 116 is responsible for the vast majority of Brucella infections in humans. B. abortus isolates formed a consistent group with bv1 and bv3 isolates in different clusters. Four MLVA11 genotypes were observed for the first time in isolates from S. Jorge and Terceira islands from Azores. From the collection of isolates analysed in this study we conclude that MLVA16(Orsay) provided a clear view of Brucella spp. population, confirming epidemiological linkage in outbreak investigations. In particular, it suggests recent and ongoing colonisation of Portugal with one B. melitensis lineage usually associated with East Mediterranean countries.

(PDF emailed within 0-6 h: $19.90)

Accession: 054208355

Download citation: RISBibTeXText

PMID: 22905141

DOI: 10.1371/journal.pone.0042514


Related references

Phage typing reactions on brucella species brucella abortus brucella neotomae brucella suis brucella melitensis brucella ovis brucella canis. Applied Microbiology 16(8): 1179-1190, 1968

MLVA genotyping of Brucella melitensis and Brucella abortus isolates from different animal species and humans and identification of Brucella suis vaccine strain S2 from cattle in China. Plos One 8(10): E76332, 2014

Genotypic Expansion Within the Population Structure of Classical Brucella Species Revealed by MLVA16 Typing of 1404 Brucella Isolates From Different Animal and Geographic Origins, 1974-2006. Frontiers in Microbiology 9: 1545, 2018

Comparison in cattle of complement fixation tests with creolysed soluble antigen (Brucella melitensis and Brucella abortus) and Brucella infecting strains by serological typing. 1985

Utilization of brucella bacterio phage tbilisi for brucella differentiation human brucella abortus brucella melitensis. Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 45(2): 119-124, 1968

Vaccination with Brucella abortus rough mutant RB51 protects BALB/c mice against virulent strains of Brucella abortus, Brucella melitensis, and Brucella ovis. Infection and Immunity 62(11): 4990-4996, 1994

Molecular prevalence of putative virulence-associated genes in Brucella melitensis and Brucella abortus isolates from human and livestock specimens in Iran. Microbial Pathogenesis 105: 334-339, 2017

Comparison of the efficacy of Brucella abortus strain RB51 and Brucella melitensis Rev. 1 live vaccines against experimental infection with Brucella melitensis in pregnant ewes. Revue Scientifique et Technique 20(3): 741-747, 2001

High susceptibility of human dendritic cells to invasion by the intracellular pathogens Brucella suis, Brucella abortus, and Brucella melitensis. Infection and Immunity 73(12): 8418-8424, 2005

Morphological, biochemical and serological characters of a strain of Brucella canis in comparison to strains of Brucella abortus, Brucella melitensis and Brucella ovis. Archivio veterinario italiano, 29(5-6): 149-151, 1978

Brucella suis s2 brucella melitensis rev. 1 and brucella abortus s19 living vaccines residual virulence and immunity induced against three brucella spp challenge strains in mice. Vaccine 8(5): 462-468, 1990

Occurrence of antibodies against brucella brucella abortus brucella melitensis brucella canis in serum samples of cloven hoofed game of west germany. Zeitschrift fuer Jagdwissenschaft 27(3): 191-195, 1981

Comparison of Brucella abortus and Brucella melitensis antigens for the Rose Bengal plate test on sera from cattle infected with Brucella abortus biovar-5. Veterinary record, 117(15): 385-386, 1985

Brucella suis S2, brucella melitensis Rev. 1 and Brucella abortus S19 living vaccines: residual virulence and immunity induced against three Brucella species challenge strains in mice. Vaccine 8(5): 462-468, 1990

A serological comparison of complement fixation reactions using brucella abortus and brucella melitensis antigens in brucella abortus infected cattle. Onderstepoort Journal of Veterinary Research 58(2): 111-114, 1991