+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Mapping of the receptor-binding domain and amino acids critical for attachment in the spike protein of avian coronavirus infectious bronchitis virus

Mapping of the receptor-binding domain and amino acids critical for attachment in the spike protein of avian coronavirus infectious bronchitis virus

Virology 448: 26-32

The infection of the avian coronavirus infectious bronchitis virus (IBV) is initiated by the binding of the spike glycoprotein S to sialic acids on the chicken host cell. In this study we identified the receptor-binding domain (RBD) of the spike of the prototype IBV strain M41. By analyzing the ability of recombinantly expressed chimeric and truncated spike proteins to bind to chicken tissues, we demonstrate that the N-terminal 253 amino acids of the spike are both required and sufficient for binding to chicken respiratory tract in an α-2,3-sialic acid-dependent manner. Critical amino acids for attachment of M41 spike are present within the N-terminal residues 19-69, which overlap with a hypervariable region in the S1 gene. Our results may help to understand the differences between IBV S1 genotypes and the ultimate pathogenesis of IBV in chickens.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 054257014

Download citation: RISBibTeXText

PMID: 24314633

DOI: 10.1016/j.virol.2013.09.018

Related references

Coronavirus infectious bronchitis virus partial amino terminal sequencing of spike polypeptide s 2 identifies the sequence arginylarginylphenylalanylarginylarginine at the cleavage site of the spike precursor propolypeptide of infectious bronchitis virus strains beaudette and m 41. Virus Research 4(2): 133-144, 1986

Coronavirus avian infectious bronchitis virus structural characterization of the spike protein. Journal of General Virology 64(12): 2577-2584, 1983

Heat shock protein 70 in lung and kidney of specific-pathogen-free chickens is a receptor-associated protein that interacts with the binding domain of the spike protein of infectious bronchitis virus. Archives of Virology 162(6): 1625-1631, 2017

A single amino acid mutation in the spike protein of coronavirus infectious bronchitis virus hampers its maturation and incorporation into virions at the nonpermissive temperature. Virology 326(2): 288-298, 2004

Identification of residues in the receptor-binding domain (RBD) of the spike protein of human coronavirus NL63 that are critical for the RBD-ACE2 receptor interaction. Journal of General Virology 89(Pt 4): 1015-1024, 2008

Heparan sulfate is a selective attachment factor for the avian coronavirus infectious bronchitis virus Beaudette. Avian Diseases 51(1): 45-51, 2007

Cryo-EM structure of infectious bronchitis coronavirus spike protein reveals structural and functional evolution of coronavirus spike proteins. Plos Pathogens 14(4): E1007009, 2018

The Infectious Bronchitis Coronavirus Envelope Protein Alters Golgi pH To Protect the Spike Protein and Promote the Release of Infectious Virus. Journal of Virology 93(11):, 2019

Localization of neutralizing epitopes and the receptor-binding site within the amino-terminal 330 amino acids of the murine coronavirus spike protein. Journal of Virology 68(9): 5403-5410, 1994

Characterisation of the RNA binding properties of the coronavirus infectious bronchitis virus nucleocapsid protein amino-terminal region. Febs Letters 580(25): 5993-5998, 2006

A single amino acid substitution (R441A) in the receptor-binding domain of SARS coronavirus spike protein disrupts the antigenic structure and binding activity. Biochemical and Biophysical Research Communications 344(1): 106-113, 2006

Sialic acid binding properties of soluble coronavirus spike (S1) proteins: differences between infectious bronchitis virus and transmissible gastroenteritis virus. Viruses 5(8): 1924-1933, 2013

Coronavirus multiplication strategy. II. Mapping the avian infectious bronchitis virus intracellular RNA species to the genome. Journal of Virology 36(2): 440-449, 1980

Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism. Journal of Virology 77(16): 9084-9089, 2003

Cloning and sequencing of the gene encoding the spike protein of the coronavirus infectious bronchitis virus. Journal of General Virology 66(4): 719-726, 1985