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Mapping protein-protein interactions with phage-displayed combinatorial peptide libraries



Mapping protein-protein interactions with phage-displayed combinatorial peptide libraries



Current Protocols in Cell Biology Chapter 17: Unit 17.4



This unit describes the process and analysis of affinity selecting bacteriophage M13 from libraries displaying combinatorial peptides fused to either a minor or major capsid protein. Direct affinity selection uses target protein bound to a microtiter plate followed by purification of selected phage by ELISA. Alternatively, there is a bead-based affinity selection method. These methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus sequence to search proteomic databases for putative interacting proteins.

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Accession: 054257162

Download citation: RISBibTeXText

PMID: 18228422

DOI: 10.1002/0471143030.cb1704s17


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