+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Mechanism of villous atrophy in celiac disease: role of apoptosis and epithelial regeneration

Mechanism of villous atrophy in celiac disease: role of apoptosis and epithelial regeneration

Archives of Pathology and Laboratory Medicine 137(9): 1262-1269

The data on status of apoptosis in patients with celiac disease are conflicting. Furthermore, complex interaction between intrinsic and common apoptotic pathways, apoptotic inhibitors, and epithelial cell proliferation is largely unclear for patients with celiac disease. To determine the role of apoptosis and epithelial cell regeneration in celiac disease. Twenty-five treatment-naïve patients with celiac disease and 6 patients with functional dyspepsia, as controls, were included and duodenal biopsy specimens from all were subjected to immunohistochemistry with markers of intrinsic apoptotic pathway (AIF, H2AX, p53), common pathway (CC3, M30), apoptotic inhibitors (XIAP, Bcl2), and epithelial proliferation (Ki-67). Apoptotic and proliferation indices were calculated. Expression of end-apoptotic products, that is, H2AX in the cell nuclei (P = .01) and M30 in the cell cytoplasm (P < .01), was significantly upregulated in celiac disease in comparison to controls. Cleaved caspase-3 was also upregulated in villous cytoplasm in celiac disease. Apoptotic inhibitor Bcl2 was significantly down-regulated in celiac disease in comparison to controls. In addition, Ki-67 proliferation index was upregulated both in the crypts and villous mucosal epithelium in comparison to the crypts of the controls. Treatment-naïve patients with celiac disease have significantly higher level of apoptosis that involves both the common and intrinsic apoptotic pathways. Increased apoptosis and unequaled cell regeneration in crypts probably results in villous atrophy. Down-regulation of apoptotic inhibitors in treatment-naïve celiac disease imparts an additional pro-apoptotic effect.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 054296849

Download citation: RISBibTeXText

PMID: 23991739

DOI: 10.5858/arpa.2012-0354-OA

Related references

A direct role for NKG2D/MICA interaction in villous atrophy during celiac disease. Immunity 21(3): 367-377, 2004

Role of the "immersion technique" in diagnosing celiac disease with villous atrophy limited to the duodenal bulb. Journal of Clinical Gastroenterology 41(6): 571-575, 2007

Screening for celiac disease in Down's syndrome patients revealed cases of subtotal villous atrophy without typical for celiac disease HLA-DQ and tissue transglutaminase antibodies. World Journal of Gastroenterology 12(9): 1430-1434, 2006

Nutritional status of newly diagnosed celiac disease patients before and after the institution of a celiac disease diet--association with the grade of mucosal villous atrophy. American Journal of Clinical Nutrition 67(3): 482-487, 1998

Patients of celiac disease with mild villous atrophy are clinically similar to those with moderate to severe atrophy. Indian Journal of Gastroenterology 32(6): 413-414, 2014

Decreased expression of protease inhibitor 9, a granzyme B inhibitor, in celiac disease: a potential mechanism in enterocyte destruction and villous atrophy. International Journal of Immunopathology and Pharmacology 26(4): 897-905, 2014

Total villous atrophy mesenteric lymph node cavitation splenic atrophy a special form of adult celiac disease a new case. Annales De Pathologie: 251-256, 1983

Total villous atrophy, mesenteric lymph-node cavitation, splenic atrophy. An unusual form of celiac disease in adults, apropos of a new case. Annales de Pathologie 3(3): 251-256, 1983

Villous atrophy and nutritional status in celiac disease. American Journal of Clinical Nutrition 69(3): 573-575, 1999

Chronic diarrhea because of villous atrophy unrelated to celiac disease. Endoscopy 47 Suppl 1 Uctn: E71-E72, 2016

Celiac disease without villous atrophy: revision of criteria called for. Digestive Diseases and Sciences 46(4): 879-887, 2001

Celiac disease without villous atrophy in children: a prospective study. Journal of Pediatrics 157(3): 373-80, 380.E1, 2010

Association of the FAS 670 gene polymorphism with villous atrophy in celiac disease. Gastroenterology. 122(4 Suppl. 1): A-181, Il, 2002

Patchy villous atrophy of the duodenum in childhood celiac disease. Journal of Pediatric Gastroenterology and Nutrition 38(2): 204-207, 2004

Celiac disease: anti-endomysial antibody versus villous atrophy. Indian Journal of Gastroenterology 25(6): 317-318, 2007