+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Microarrays of recombinant Hevea brasiliensis proteins: a novel tool for the component-resolved diagnosis of natural rubber latex allergy



Microarrays of recombinant Hevea brasiliensis proteins: a novel tool for the component-resolved diagnosis of natural rubber latex allergy



Journal of Investigational Allergology and Clinical Immunology 20(2): 129-138



Component-resolved diagnosis using microarray technology has recently been introduced in clinical allergology, but its applicability in patients with natural rubber latex (NRL) allergy has not been investigated. To evaluate the utility of microarray-based immunoglobulin (Ig) E detection in the diagnostic workup of NRL allergy and to compare this new diagnostic tool with established methods of NRL-specific IgE detection. We investigated 52 adults with immediate-type NRL allergy and 50 control patients. Determination of specific serum IgE against 8 recombinant Hevea brasiliensis allergen components was performed using a customized allergen microarray and a conventional fluorescence enzyme immunoassay (FEIA). The panel of microarrayed allergen components was shown to represent a comprehensive repertoire of clinically relevant NRL proteins. NRL-specific IgE recognition patterns and sensitization rates determined by microarray analysis were similar to those obtained by conventional FEIA. The diagnostic sensitivity rates of combined single-component data were not significantly different for the respective recombinant test system, whereas the sensitivity level of extract-based FEIA analysis was markedly higher. The current study provides evidence that microarrays of recombinant NRL allergen components are a suitable new tool for the diagnosis of NRL-specific sensitization.They show performance characteristics comparable to those of current diagnostic tests and could be indicated in small children in whom only limited blood volumes are obtainable. Further large-scale studies in unselected patient populations and in high-risk groups are warranted before the microarray can be introduced into routine management of patients with NRL allergy.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 054369207

Download citation: RISBibTeXText

PMID: 20461967


Related references

Allergenicity of Hev b 13, a major esterase allergen in natural rubber latex (Hevea brasiliensis) allergy, does not only depend on its carbohydrate moiety. Molecular Immunology 47(4): 871-877, 2010

In vivo sensitization to purified Hevea brasiliensis proteins in health care workers sensitized to natural rubber latex. Journal of Allergy and Clinical Immunology 111(3): 610-616, 2003

Micro-organisms in latex and natural rubber coagula of Hevea brasiliensis and their impact on rubber composition, structure and properties. Journal of Applied Microbiology 117(4): 921-929, 2014

Absence of cross-reactivity of IgE antibodies from subjects allergic to Hevea brasiliensis latex with a new source of natural rubber latex from guayule (Parthenium argentatum). Journal of Allergy and Clinical Immunology 98(5, part 1): 895-902, 1996

Rubber elongation factor (REF), a major allergen component in Hevea brasiliensis latex has amyloid properties. Plos one 7(10): E48065, 2012

Technological properties of latex and natural rubber of Hevea brasiliensis clones. Scientia Agricola 62(2): 122-126, 2005

Spectrographic determination of calcium in latex of natural rubber (Hevea brasiliensis). Applied spectroscopy: 24 (6) 583-587, 1970

Diagnosis of Chenopodium album allergy with a cocktail of recombinant allergens as a tool for component-resolved diagnosis. Molecular Biology Reports 39(3): 3169-3178, 2012

Diagnostic sensitivity of puncture skin testing with isolated native and recombinant proteins from Hevea brasiliensis for the diagnosis of latex hypersensitivity in healthcare workers. Journal of Allergy & Clinical Immunology 109(1 Suppl.): S257, 2002

Identification, cloning, and sequence of a major allergen (Hev b5) from natural rubber latex (Hevea brasiliensis). Journal of biological chemistry 271(41): 25394-25399, 1996

Natural rubber latex synthesized in later vessel of Hevea brasiliensis can be fractionated into three fractions upon ultracentrifugation. Plant Physiology (Rockville) 114(3 Suppl. ): 72, 1997