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Microfluidic whole genome amplification device for single cell sequencing

Microfluidic whole genome amplification device for single cell sequencing

Analytical Chemistry 86(19): 9386-9390

We developed a microfluidic device to perform multiplex single-cell whole-genome amplification (WGA) using multiple annealing and looping-based amplification cycles (MALBAC). This device, made of polydimethylsiloxane (PDMS), allows us to monitor the whole process of cell loading and single-cell WGA for sequencing. We show that the genome coverage of MALBAC amplifications is reproducible between chambers on a single chip and between different chips, which enables data normalization using standard samples to accurately identify copy number variations (CNVs). This device provides an easy-to-operate approach to perform single cell sequencing library preparation with minimum hands-on time. It reduces the requirement of manual expertise as well as the risk of contamination, which is essential in future applications especially the medical diagnosis.

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Accession: 054375259

Download citation: RISBibTeXText

PMID: 25233049

DOI: 10.1021/ac5032176

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