+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Micropropagation of Gerbera (Gerbera jamesonii Bolus)

Micropropagation of Gerbera (Gerbera jamesonii Bolus)

Methods in Molecular Biology 11013: 305-316

ISSN/ISBN: 1064-3745

PMID: 23179709

DOI: 10.1007/978-1-62703-074-8_24

Gerbera (Gerbera jamesonii Bolus) is one of the most popular ornamental flowers worldwide and used both as cut flower and potted plant. Some of them show excellent agronomic characters such as color, floral diameter, stem length, and vigor, which make this plant of commercial importance. Conventionally, multiplication is done through seeds or rhizome cuttings. Rapid multiplication of elite cultivars of Gerbera, with improved agronomic traits, has been achieved by using both direct and indirect tissue culture methods. Direct shoot regeneration was accomplished from stem apices on MS medium supplemented with 1 mg/L 6-benzyladenine (BA) and 1 mg/L kinetin. Indirect shoot induction succeeded from callus differentiation has been achieved on MS medium containing 2 mg/L 2,4-dichlorophenoxyacetic acid, 0.5 mg/L indole-3-acetic acid, and 2 mg/L BA. The in vitro shoots, 4-5 cm long, were rooted by quick dipping the shoot bases for 3-5 s in 2,000 mg/L indole-3-butyric acid solution followed by transfer to the pots containing farmyard manure, soil, and sand (1:1:1 by volume). Initially, in vitro plantlets were covered with glass jars to maintain a high relative humidity (85-90%). As soon as new shoot growth begins, relative humidity is decreased by exposing them to the open environmental conditions prior transferring to the glasshouse. Indirect shoot regeneration increased the frequency of somaclonal variations. The selected somaclones were used in developing new and novel cultivars.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 054377807

Download citation: RISBibTeXText

Related references

Zur Differentialdiagnose des pibzlichen Welkeerreger-komplexes bei Gerbera jamesonii Bolus - Diagnose différentielle des champignons phytopathogènes sur G.j - On the differential diagnosis of fungal wilt pathogens in Gerbera jamesonii Bolus. Nachrichtenbl. Pflanzenschutz Ddr, Berlin 39(1): 14-15, 1985

Micropropagation of Gerbera jamesonii (Bolus) on different culture media. Advances in Plant Sciences 19(1): 19-22, 2006

In vitro formation of gerbera (Gerbera jamesonii Bolus) plantlets from capitulum explants. Propagation of Ornamental Plants 7(1): 37-42, 2007

Flower yield evaluation some of gerbera cultivars and clones (Gerbera jamesonii Bolus). Prace Poznanskie Towarzystwo Przyjaciol Nauk Wydzial Nauk Rolniczych i Lesnych 5(55): 153-159, 1983

Effect of the date of planting vegetatively propagated gerbera (Gerbera jamesonii Bolus). Sbornik, UVTIZ, Zahradnictvi 12(1): 67-72, 1985

Commercial multiplication of gerbera (Gerbera jamesonii Bolus) through in vitro shoot tip culture. Journal of Ornamental Horticulture New Series 6(4): 303-309, 2003

Stability and association among economical characters in gerbera (Gerbera jamesonii Bolus ex Hooker F.). Journal of Ornamental Horticulture New Series 8(3): 191-195, 2005

In vitro culture of gerbera (Gerbera jamesonii H. Bolus) and its acclimation in greenhouse. Bioagro 12(3): 75-80, 2000

Performance of different varieties of gerbera (Gerbera jamesonii H. Bolus) under polyhouse conditions. Advances in Plant Sciences 18(1): 269-272, 2005

Carbohydrate contents of gerbera (Gerbera jamesonii Bolus) inflorescence during senescence. Prace Instytutu Sadownictwa i Kwiaciarstwa w Skierniewicach, B 4: 113-118, 1979

Performance of gerbera (Gerbera jamesonii Bolus ex Hooker F.) cultivars under fan and pad cooled greenhouse conditions. Research on Crops 13(1): 299-301, 2012

Evaluation of gerbera (Gerbera jamesonii Bolus ex Hooker F.) cultivars under sub-tropical hills of Meghalaya. Journal of Ornamental Horticulture New Series 8(3): 212-215, 2005

Effect of spacing on yield and quality of gerbera (Gerbera jamesonii Bolus ex hoof. f) under polyhouse. Journal of Ornamental Horticulture New Series 8(1): 62-64, 2005

Evaluation of the genetic fidelity of in vitro-propagated gerbera (Gerbera jamesonii Bolus) using DNA-based markers. Plant Cell, Tissue and Organ Culture 104(1): 131-135, 2011

Efecto del nitrógeno en los parámetros fotosintéticos y de producción del cultivo de la gerbera (Gerbera jamesonii H. Bolus ex Hook. f.). Acta Agronómica 65(3): 255-260, 2016