+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Mitochondrial atp9 genes from petaloid male-sterile and male-fertile carrots differ in their status of heteroplasmy, recombination involvement, post-transcriptional processing as well as accumulation of RNA and protein product

Mitochondrial atp9 genes from petaloid male-sterile and male-fertile carrots differ in their status of heteroplasmy, recombination involvement, post-transcriptional processing as well as accumulation of RNA and protein product

Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 127(8): 1689-1701

ISSN/ISBN: 0040-5752

PMID: 24913361

DOI: 10.1007/s00122-014-2331-x

Petaloid cytoplasmic male-sterile carrots exhibit overexpression of the mitochondrial atp9 genes which is associated with specific features in organization and expression of these sequences. In carrots, the Sp-cytoplasm causes transformation of stamens into petal-like organs, while plants carrying normal N-cytoplasm exhibit normal flower morphology. Our work was aimed at characterization of distinct features both cytoplasms display with respect to organization and expression of the mitochondrial atp9 genes. We show that two carrot atp9 genes, previously reported as cytoplasm-specific, in fact occur in heteroplasmic condition. In the Sp-cytoplasm the atp9-1 version dominates over atp9-3, while in N-cytoplasmic plants this proportion is reversed. Herein, we also indicate the presence and recombination activity of a 130-/172-bp sequence repeat which likely shaped the present organization of carrot atp9 loci. Furthermore, cDNA sequence examination revealed that the atp9 open reading frames (ORFs) were C to U edited in 4 nucleotide positions. One of the editing events turns a glutamine triplet into the stop codon, thereby equalizing ORFs of atp9-1 and atp9-3. A certain fraction of partially edited molecules was identified-they all represented the atp9-3 sequence. In either Sp- or N-cytoplasmic plants multiple 5' transcript termini were observed. Of these, the ones mapping more distantly from the atp9 ORF were more pronounced in case of petaloid accessions. It was also shown that despite comparable copy number of the genomic atp9 sequences, the level of the respective mRNAs was approximately 3 times higher in case of petaloid carrots. The latter fact corresponded to the elevated content of the ATP9 protein in plants carrying Sp-cytoplasm. The semi-fertile phenotype of such plants is associated with a drop in ATP9 accumulation.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 054402668

Download citation: RISBibTeXText

Related references

A fertile revertant from petaloid cytoplasmic male-sterile carrot has a rearranged mitochondrial genome. Theoretical and applied genetics 97(3): 450-455, 1998

Analysis of mitochondrial DNA heteroplasmy of fertile and male-sterile sugar beet plants Beta vulgaris. Russian Journal of Genetics: Applied Research 2(1): 53-57, 2012

Analysis of a fertile revertant from petaloid cytoplasmic male sterile carrot. Plant Physiology 99(1 Suppl): 92, 1992

Mitochondrial gene expression in developing male gametophytes of male-fertile and S male-sterile maize. Sexual Plant Reproduction 11(6): 323-330, 1999

Butinage préférentiel de l'abeille sur les fleurs mâle-stérile et mâle-fertile de l'abricotier japonais (Prunus mume) - Preferential honeybee foraging between male-fertile and male-sterile flower of Japanese apricot (Prunus mume). Honeybee Science 4(1): 1-8, 1983

Floral development in petaloid cytoplasmic male sterile and fertile lines of carrot. Plant Physiology 99(1 Suppl): 53, 1992

Selection of petaloid type male sterile lines with white petal color in carrots. Journal of the Korean Society for Horticultural Science 36(1): 10-20, 1995

Observations on flowering habit and seed yield of petaloid type male-sterile carrots. Zoldsegtermesztesi Kutato Intezet Bulletinje 22: 51-57, 1989

Phylogenetic relationships among fertile and petaloid male-sterile accessions of carrot, Daucus carota L. Theoretical and applied genetics 96(6-7): 928-932, 1998

Genetic variation of petaloid male-sterile cytoplasm of carrots revealed by sequence-tagged sites (STSs). Theoretical and Applied Genetics 99(5): 837-843, 1999

A Reevaluation of Rice Mitochondrial Evolution Based on the Complete Sequence of Male-Fertile and Male-Sterile Mitochondrial Genomes. Plant Physiology 158(2): 996-1017, 2012

A reevaluation of rice mitochondrial evolution based on the complete sequence of male-fertile and male-sterile mitochondrial genomes. Plant Physiology 158(2): 996-1017, 2012

Sequence and Expression of a Fused Mitochondrial Gene, Associated with Petunia Cytoplasmic Male Sterility, Compared with Normal Mitochondrial Genes in Fertile and Sterile Plants. Philosophical Transactions Biological Sciences 319(1193): 199-208, 1988

Sequence and expression of a fused mitochondrial gene, associated with Petunia cytoplasmic male sterility, compared with normal mitochondrial genes in fertile and sterile plants. Mitochondrial Biogenesis. Discussion (1987) 319(1193): 199-208, 1988

Sequence of expression of a fused mitochondrial gene associated with petunia cytoplasmic male sterility compared with normal mitochondrial genes in fertile and sterile plants. Philosophical Transactions of the Royal Society of London B Biological Sciences 319(1193): 199-208, 1988