+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Mitochondrial proteins differential expression during honeybee (Apis mellifera L.) queen and worker larvae caste determination



Mitochondrial proteins differential expression during honeybee (Apis mellifera L.) queen and worker larvae caste determination



Journal of Proteome Research 10(9): 4263-4280



Despite their similar genetic makeup, honeybee (A. mellifera) queens and workers show alternative morphologies driven by nutritional difference during the larval stage. Although much research have been done to investigate the causes of honeybee caste polymorphism, information at subcellular protein levels is limited. We analyzed queen- and worker-destined larvae mitochondrial proteome at three early developmental stages using combinations of differential centrifugation, two-dimensional electrophoresis, mass spectrometry, bioinformatics, and quantitative real time PCR. In total, 67, 69, and 97 protein spots were reproducibly identified as mitochondrial proteins at 72, 96, and 120 h, respectively. There were significant qualitative and quantitative protein expression differences between the two castes at three developmental stages. In general, the queen-destined larvae up-regulated large proportions of proteins at all of the developmental stages and, in particular, 95% at 72 h. An overwhelming majority of the queen larvae up-regulated proteins were physiometabolic-enriched proteins (metabolism of carbohydrate and energy, amino acid, and fatty acid) and involved in protein folding, and this was further verified by functional enrichment and biological interaction network analyses as a direct link with metabolic rates and cellular responses to hormones. Although wide-ranging mitochondrial proteomes participate to shape the metabolic, physiologic, and anatomic differences between the two castes at 72 h, physiometabolic-enriched proteins were found as the major modulators of the profound marking of this caste differentiation. Owing to nutritional difference, prospective queen larvae showed enhanced growth, and this was manifested through the overexpression of metabolic enzymes. Differently from similar studies targeting the causes of honeybee caste polymorphism, this subcellular level study provides an in-depth insight into mitochondrial proteins-mediated caste polymorphism and greatly improves protein coverage involved during honeybee caste determination. Hence, it is a major step forward in the analysis of the fundamental causes of honeybee caste pathway decision and greatly contributes to the knowledge of honeybee biology. In particular, the consistency between the 22 proteins and mRNA expressions provides us important target genes for the reverse genetic analysis of caste pathway modulation through RNA interference.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 054404314

Download citation: RISBibTeXText

PMID: 21751814

DOI: 10.1021/pr200473a


Related references

Differential expressions of nuclear proteomes between honeybee (Apis mellifera L.) Queen and Worker Larvae: a deep insight into caste pathway decisions. Journal of Proteome Research 11(2): 1317-1329, 2012

Differential expression of mitochondrial genes between queens and workers during caste determination in the honeybee Apis mellifera. Journal of Experimental Biology 202(Pt 8): 929-938, 1999

Differential feeding of worker larvae affects caste characters in the Cape honeybee, Apis mellifera capensis. Behavioral Ecology and Sociobiology ober; 54(6): 555-561, 2003

Differential Feeding of Worker Larvae Affects Caste Characters in the Cape Honeybee, Apis mellifera capensis. Behavioral Ecology and Sociobiology 54(6): 555-561, 2003

The insulin signaling pathway in honey bee (Apis mellifera) caste development - differential expression of insulin-like peptides and insulin receptors in queen and worker larvae. Journal of Insect Physiology 54(6): 1064-1071, 2008

Research progress on the queen-worker caste differentiation in the honeybee (Apis mellifera). Entomological Knowledge 39(3): 176-181, 2002

Differential protein expression in honeybee (Apis mellifera L.) larvae: underlying caste differentiation. Plos one 5(10): E13455, 2010

Differential Protein Expression in Honeybee Apis mellifera L Larvae Underlying Caste Differentiation. PLoS ONE 5(10): e13455, 2010

Uncovering the Changing Gene Expression Profile of Honeybee ( Apis mellifera ) Worker Larvae Transplanted to Queen Cells. Frontiers in Genetics 9: 416, 2018

Differential gene expression in the mandibular glands of queen and worker honeybees, Apis mellifera L.: implications for caste-selective aldehyde and fatty acid metabolism. Insect Biochemistry and Molecular Biology 39(10): 661-667, 2009

A comparison of honeybee (Apis mellifera) queen, worker and drone larvae by RNA-Seq. Insect Science 26(3): 499-509, 2019

The rate of growth of worker, drone and queen larvae of the honeybee, Apis mellifera Linn. Jour Econ Ent 23(2): 447-453, 1930

Insights into the dynamics of hind leg development in honey bee (Apis mellifera L.) queen and worker larvae - A morphology/differential gene expression analysis. Genetics and Molecular Biology 38(3): 263-277, 2015

Differential antennal proteome comparison of adult honeybee drone, worker and queen (Apis mellifera L.). Journal of Proteomics 75(3): 756-773, 2012

Isolation and characterization of proteins and lipids from honeybee Apis mellifera L queen larvae and royal jelly. Food Research International 54(1): 330-337, 2013