+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Mitochondrial transcription factor A regulates mitochondrial transcription initiation, DNA packaging, and genome copy number

Mitochondrial transcription factor A regulates mitochondrial transcription initiation, DNA packaging, and genome copy number

Biochimica et Biophysica Acta 1819(9-10): 921-929

Mitochondrial transcription factor A (mtTFA, mtTF1, TFAM) is an essential protein that binds mitochondrial DNA (mtDNA) with and without sequence specificity to regulate both mitochondrial transcription initiation and mtDNA copy number. The abundance of mtDNA generally reflects TFAM protein levels; however, the precise mechanism(s) by which this occurs remains a matter of debate. Data suggest that the usage of mitochondrial promoters is regulated by TFAM dosage, allowing TFAM to affect both gene expression and RNA priming for first strand mtDNA replication. Additionally, TFAM has a non-specific DNA binding activity that is both cooperative and high affinity. TFAM can compact plasmid DNA in vitro, suggesting a structural role for the non-specific DNA binding activity in genome packaging. This review summarizes TFAM-mtDNA interactions and describes an emerging view of TFAM as a multipurpose coordinator of mtDNA transactions, with direct consequences for the maintenance of gene expression and genome copy number. This article is part of a Special Issue entitled: Mitochondrial Gene Expression.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 054404663

Download citation: RISBibTeXText

PMID: 22465614

DOI: 10.1016/j.bbagrm.2012.03.002

Related references

Mitochondrial Lon protease regulates mitochondrial DNA copy number and transcription by selective degradation of mitochondrial transcription factor A (TFAM). Proceedings of the National Academy of Sciences of the United States of America 107(43): 18410-5, 2010

Drosophila mitochondrial transcription factor B2 regulates mitochondrial DNA copy number and transcription in schneider cells. Journal of Biological Chemistry 279(26): 26900-5, 2004

Drosophila mitochondrial transcription factor B1 modulates mitochondrial translation but not transcription or DNA copy number in Schneider cells. Journal of Biological Chemistry 280(17): 16815-16820, 2005

Mitochondrial transcription factor A regulates mtDNA copy number in mammals. Human Molecular Genetics 13(9): 935-944, 2004

Transient overexpression of mitochondrial transcription factor A (TFAM) is sufficient to stimulate mitochondrial DNA transcription, but not sufficient to increase mtDNA copy number in cultured cells. Nucleic Acids Research 32(20): 6015-6027, 2004

P53 increase mitochondrial copy number via up-regulation of mitochondrial transcription factor A in colorectal cancer. Oncotarget 7(46): 75981-75995, 2016

Functional domains of chicken mitochondrial transcription factor A for the maintenance of mitochondrial DNA copy number in lymphoma cell line DT40. Journal of Biological Chemistry 278(33): 31149-31158, 2003

Drosophila nuclear factor DREF regulates the expression of the mitochondrial DNA helicase and mitochondrial transcription factor B2 but not the mitochondrial translation factor B1. Biochimica et Biophysica Acta 1829(10): 1136-1146, 2013

Mitochondrial transcription termination factor 2 binds to entire mitochondrial DNA and negatively regulates mitochondrial gene expression. Acta Biochimica et Biophysica Sinica 43(6): 472-479, 2011

Proliferation of mitochondria in chronically stimulated rabbit skeletal muscle--transcription of mitochondrial genes and copy number of mitochondrial DNA. Journal of Bioenergetics and Biomembranes 32(6): 627-634, 2000

Characterization of transcription initiation sites on the soybean mitochondrial genome allows identification of a transcription-associated sequence motif. Molecular and General Genetics 228(3): 345-355, 1991

Human Mitochondrial Transcription Factor B2 Is Required for Promoter Melting during Initiation of Transcription. Journal of Biological Chemistry 292(7): 2637-2645, 2017

Human mitochondrial transcription factor A and promoter spacing integrity are required for transcription initiation. Biochimica Et Biophysica Acta. 1271(1): 127-134, 1995

Chromosome 21-derived hsa-miR-155-5p regulates mitochondrial biogenesis by targeting Mitochondrial Transcription Factor A (TFAM). Biochimica et Biophysica Acta 1852(7): 1420-1427, 2015

MicroRNA-494 regulates mitochondrial biogenesis in skeletal muscle through mitochondrial transcription factor A and Forkhead box j3. American Journal of Physiology. Endocrinology and Metabolism 303(12): E1419-E1427, 2012