+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Mitogen-activated protein kinase 6 controls root growth in Arabidopsis by modulating Ca2+ -based Na+ flux in root cell under salt stress



Mitogen-activated protein kinase 6 controls root growth in Arabidopsis by modulating Ca2+ -based Na+ flux in root cell under salt stress



Journal of Plant Physiology 171(5): 26-34



Little is known about the role of mitogen-activated protein kinase 6 (MPK6) in Na(+) toxicity and inhibition of root growth in Arabidopsis under NaCl stress. In this study, we found that root elongation in seedlings of the loss-of-function mutants mpk6-2 and mpk6-3 was less sensitive to NaCl or Na-glutamate, but not to KCl or mannitol, as compared with that of wild-type (WT) seedlings. The less sensitive characteristic was eliminated by adding the Ca(2+) chelator EGTA or the Ca(2+) channel inhibitor LaCl3, but not the Ca(2+) ionophore A23187. This suggested that the tolerance of mpk6 to Na(+) toxicity was Ca(2+)-dependent. We measured plasma membrane (PM) Na(+)-conducted currents (NCCs) in root cells. Increased concentrations of NaCl increased the inward NCCs while decreased the outward NCCs in WT root cells, attended by a positive shift in membrane potential. In mpk6 root cells, NaCl significantly increased outward but not inward NCCs, accompanied by a negative shift in membrane potential. That is, mpk6 decreased NaCl-induced the Na(+) accumulation by modifying PM Na(+) flux in root cells. Observations of aequorin luminescence revealed a NaCl-induced increase of cytosolic Ca(2+) in mpk6 root cells, resulting from PM Ca(2+) influx. An increase of cytosolic Ca(2+) was required to alleviate the NaCl-increased Na(+) content and Na(+)/K(+) ratio in mpk6 roots. Together, these results show that mpk6 accumulated less Na(+) in response to NaCl because of the increased cytosolic Ca(2+) level in root cells; thus, its root elongation was less inhibited than that of WT by NaCl.

(PDF emailed within 0-6 h: $19.90)

Accession: 054404897

Download citation: RISBibTeXText

PMID: 24484955

DOI: 10.1016/j.jplph.2013.09.023


Related references

A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America, 93(2): 765-769, 1996

Involvement of YODA and mitogen activated protein kinase 6 in Arabidopsis post-embryogenic root development through auxin up-regulation and cell division plane orientation. New Phytologist 203(4): 1175-1193, 2015

Tumor necrosis factor signaling to stress-activated protein kinase /Jun NH2-terminal kinase and p38 Germinal center kinase couples TRAF2 to mitogen-activated protein kinase/ERK kinase kinase 1 and SAPK while receptor interacting protein associates with a mitogen-activated protein kinase kinase kinase upstream of MKK6 and p38. Journal of Biological Chemistry 273(35): 22681-22692, Aug 28, 1998

Salt stress in Arabidopsis: lipid transfer protein AZI1 and its control by mitogen-activated protein kinase MPK3. Molecular Plant 7(4): 722-738, 2015

P38 Mitogen-Activated Protein Kinase Controls NF-(Sm(BB Transcriptional Activation and Tumor Necrosis Factor Alpha Production through RelA Phosphorylation Mediated by Mitogen- and Stress-Activated Protein Kinase 1 in Response to Borrelia burgdorferi Antigens. Infection and immunity IAI 75(1): 270-277, 2007

Osmotic stress induces the activation of a mitogen- activated protein kinase and a calcium-independent protein kinase from beet root. Plant Science (Oxford) 167(3): 561-567, September, 2004

P38 mitogen-activated protein kinase controls NF-kappa B transcriptional activation and tumor necrosis factor alpha production through RelA phosphorylation mediated by mitogen- and stress-activated protein kinase 1 in response to Borrelia burgdorferi antigens. Infection and Immunity 75(1): 270-277, 2007

p38 mitogen-activated protein kinase controls NF-kappaB transcriptional activation and tumor necrosis factor alpha production through RelA phosphorylation mediated by mitogen- and stress-activated protein kinase 1 in response to Borrelia burgdorferi antigens. Infection and Immunity 75(1): 270-277, 2006

Osmotic stress induces the activation of a mitogen-activated protein kinase (MAPK) and a calcium-independent protein kinase from beet root (Beta vulgaris L.). Plant Science 167(3): 561-567, 2004

Overexpression of maize mitogen-activated protein kinase gene, ZmSIMK1 in Arabidopsis increases tolerance to salt stress. Molecular Biology Reports 37(8): 4067-4073, 2011

Tumor necrosis factor signaling to stress-activated protein kinase (SAPK)/Jun NH2-terminal kinase (JNK) and p38. Germinal center kinase couples TRAF2 to mitogen-activated protein kinase/ERK kinase kinase 1 and SAPK while receptor interacting protein associates with a mitogen-activated protein kinase kinase kinase upstream of MKK6 and p38. Journal of Biological Chemistry 273(35): 22681-22692, 1998

Arabidopsis thaliana mitogen-activated protein kinase 6 is involved in seed formation and modulation of primary and lateral root development. Journal of Experimental Botany 65(1): 169-183, 2014

Mitogen-Activated Protein Kinase 6 and Ethylene and Auxin Signaling Pathways Are Involved in Arabidopsis Root-System Architecture Alterations by Trichoderma atroviride. Molecular Plant-Microbe Interactions 28(6): 701-710, 2015

The Cotton Mitogen-Activated Protein Kinase Kinase 3 Functions in Drought Tolerance by Regulating Stomatal Responses and Root Growth. Plant & Cell Physiology 57(8): 1629-1642, 2016

Involvement of the mitogen-activated protein kinase SIMK in regulation of root hair tip growth. Embo Journal 21(13): 3296-3306, 2002