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Modulation of the activity of phosphoenolypyruvate carboxylase during potassium-induced swelling of guard-cell protoplasts of Vicia faba L. after light and dark treatments


Modulation of the activity of phosphoenolypyruvate carboxylase during potassium-induced swelling of guard-cell protoplasts of Vicia faba L. after light and dark treatments



Planta 180(2): 188-193



ISSN/ISBN: 0032-0935

PMID: 24201943

DOI: 10.2307/23380096

Phosphoenolpyruvate carboxylase (PEPCase; EC 4.1.1.31) activity was found to be modulated by light and darkness when measured in the presence of K+, which had been added to induce swelling of guard-cell protoplasts (GCPs) from Vicia faba L., whereas no modulation was detected in the absence of K+ (PEPcase activity remained constant at 1.5 .+-. 0.15 pmol PEP metabolized.cntdot.GCP-1.cntdot.h-1; subsequently, pmol GCP-1.cntdot.h-1 will be used). The activity of PEPCase increased by 100% (from 1.5 to 3 pmol.cntdot.protoplast-1.cntdot.h-1) in darkness and by 200% (from 1.7 to 5 pmol.cntdot.protoplast-1.cntdot.h-1) in light and oscillations in activity of these magnitudes were repeated at intervals of 2 min (dark) and 2.5 min (light) for a period of 10 min during K+-induced increase in the volume of GCPs. The oscillations were reflected in changes in malate-pool sizes determined in plastids, mitochondria and the supernatant fraction (consisting of the cytosol and the vacuole). Malate probably functioned as a mitochondrial substrate, thus supplying ATP for K+ uptake and the swelling of the protoplasts. On the basis of the present paper and previous results (H. Schnabl and B. Michalke 1988, Life Sci. Adv. Plant Physiol. 7, 203-207) involving adenine nucleotide-pool sizes in fractionated GCPs, a model is proposed to explain the cause-effect relationship between K+, PEPCase, the cytosolic and mitochondrial malate levels and ATP levels during the K+-induced increases of GCP volume.

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Accession: 054429176

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