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Molecular characterisation of a Cryptosporidium parvum rhoptry protein candidate related to the rhoptry neck proteins TgRON1 of Toxoplasma gondii and PfASP of Plasmodium falciparum

Molecular characterisation of a Cryptosporidium parvum rhoptry protein candidate related to the rhoptry neck proteins TgRON1 of Toxoplasma gondii and PfASP of Plasmodium falciparum

Molecular and Biochemical Parasitology 183(1): 94-99

Given the lack of knowledge on the rhoptry proteins ofCryptosporidium parvum, we searched for putative members of this protein class in the CryptoDB database using as queries knownT. gondiirhoptry molecules. We cloned aC. parvumsporozoite cDNA of 4269 bp encoding the sushi domain-containing protein cgd8_253, which shared low amino acid sequence identity, yet a highly conserved domain architecture with the rhoptry neck proteinsTgRON1 ofT. gondiiand PfASP ofPlasmodium falciparum. On denaturing and native gels, cgd8_253 migrated at approximately 15 and 1 kDa, respectively, suggesting an involvement in a multi-subunit protein complex. Immunoflorescence localised cgd8_253 to a single, elongated area anterior to sporozoite micronemes and showed protein relocation to the parasite-host cell interface in early epicellular stages. Our data strongly suggest a rhoptry localization for the newly characterised protein, which was therefore renamedCryptosporidium parvumPutative Rhoptry Protein-1 (CpPRP1).Cloning of the first putative rhoptry protein ofCryptosporidium parvum(CpPRP1)CpPRP1 is related to rhoptry neck proteins ofToxoplasma gondiandPlasmodiumspp.> CpPRP1 is implicated in a multi-subunit protein complex Localization of CpPRP1 to the apical end of sporozoites and merozoites Early in invasion CpPRP1 is relocalised to the parasite-host cell interface.

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Accession: 054434765

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PMID: 22343414

DOI: 10.1016/j.molbiopara.2012.02.004

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