Morphing hydrogel patterns by thermo-reversible fluorescence switching
Bat, E.; Lin, E.-W.; Saxer, S.; Maynard, H.D.
Macromolecular Rapid Communications 35(14): 1260-1265
2014
ISSN/ISBN: 1521-3927
PMID: 24740924
DOI: 10.1002/marc.201400160
Accession: 054465875
Stimuli responsive surfaces that show reversible fluorescence switching behavior in response to temperature changes were fabricated. Oligo(ethylene glycol) methacrylate thermoresponsive polymers with amine end-groups were prepared by atom transfer radical polymerization (ATRP). The polymers were patterned on silicon surfaces by electron beam (e-beam) lithography, followed by conjugation of self-quenching fluorophores. Fluorophore conjugated hydrogel thin films were bright when the gels were swollen; upon temperature-induced collapse of the gels, self-quenching of the fluorophores led to significant attenuation of fluorescence. Importantly, the fluorescence was regained when the temperature was cooled. The fluorescence switching behavior of the hydrogels for up to ten cycles was investigated and the swelling-collapse was verified by atomic force microscopy. Morphing surfaces that change shape several times upon increase in temperature were obtained by patterning multiple stimuli responsive polymers.