+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Nitrogen regulation involved in the accumulation of urea in Saccharomyces cerevisiae

Nitrogen regulation involved in the accumulation of urea in Saccharomyces cerevisiae

Yeast 30(11): 437-447

Rice wine is a popular traditional alcoholic drink with a long history in China. However, the presence of the potential carcinogen ethyl carbamate (EC) raises a series of food safety concerns. Although the metabolic pathway of urea (the major precusor of EC) has been characterized in Saccharomyces cerevisiae, the regulation of urea accumulation remains unclear, making the efficient elimination of urea difficult. To demonstrate the regulatory mechanisms governing urea accumulation, three key nitrogen sources that can inhibit urea utilization for a commercial S. cerevisiae strain were identified. In addition, regulators of nitrogen catabolite repression (NCR) and target of rapamycin (TOR) pathways were identified as being involved in urea accumulation by real-time quantitative PCR. Based on these results, preferred nitrogen sources were found to repress urea utilization by converting them to glutamine or glutamate. Moreover, the results indicated that the manner of urea metabolism regulation was different for two positive regulators involved in NCR; Gln3p can be retained in the cytoplasm by glutamine, while Gat1p can be retained by glutamine and glutamate. Furthermore, this was confirmed by fluorescence location detection. These new findings provide new targets for eliminating EC and other harmful nitrogen-containing compounds in fermented foods.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 054627929

Download citation: RISBibTeXText

PMID: 23996237

DOI: 10.1002/yea.2980

Related references

RAS2/PKA pathway activity is involved in the nitrogen regulation of L-leucine uptake in Saccharomyces cerevisiae. International Journal of Biochemistry & Cell Biology 29(3): 505-512, 1997

Adaptive Evolution Relieves Nitrogen Catabolite Repression and Decreases Urea Accumulation in Cultures of the Chinese Rice Wine Yeast Strain Saccharomyces cerevisiae XZ-11. Journal of Agricultural and Food Chemistry 66(34): 9061-9069, 2018

Positive regulatory elements involved in urea amidolyase and urea uptake induction in Saccharomyces cerevisiae. Current Genetics 4(1): 13-18, 1981

Plc1p, Arg82p, and Kcs1p, enzymes involved in inositol pyrophosphate synthesis, are essential for phosphate regulation and polyphosphate accumulation in Saccharomyces cerevisiae. Journal of Biological Chemistry 280(26): 25127-25133, 2005

Proprietes et controle genetiques du systeme d-accumulation des acides amines chez Saccharomyces cerevisiae Properties and genetic control of the amino acid accumulation system in Saccharomyces cerevisiae. Biochim Biophys Acta: 546-566, 1965

COT1, a gene involved in cobalt accumulation in Saccharomyces cerevisiae. Molecular and Cellular Biology 12(9): 3678-3688, 1992

Regulation of arginine and urea metabolism in saccharomyces cerevisiae. Plant Biochemical Journal 1(2): 37-52, 1974

Regulation of NAD-GDH of saccharomyces cerevisiae--role of URE2 in nitrogen regulation, identification and characterization of carbon regulation. 1991

Regulation of the urea active transporter gene (DUR3) in Saccharomyces cerevisiae. Journal of Bacteriology 175(15): 4688-4698, 1993

AGT1, encoding an a-glucoside transporter involved in uptake and intracellular accumulation of trehalose in Saccharomyces cerevisiae. Journal of Bacteriology 181(12): 30-2, 1999

An acyl-CoA:cholesterol acyltransferase (ACAT)-related gene is involved in the accumulation of triacylglycerols in Saccharomyces cerevisiae. Biochemical Society Transactions 28(6): 700-702, 2001

Genes involved in the regulation of invertase production in Saccharomyces cerevisiae. Microbiologia 10(4): 385-394, 1994

Novel genes involved in telomere length regulation in Saccharomyces cerevisiae. Yeast 20(Supplement 1): S88, 2003

Elements involved in the regulation of the met25 gene of saccharomyces cerevisiae. Yeast 6(SPEC ISSUE): S325, 1990

Characterization of Gac1p, a regulatory subunit of protein phosphatase type I involved in glycogen accumulation in Saccharomyces cerevisiae. MGG Molecular Genetics & Genomics 265(4): 622-635, 2001