EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Nitrophorin 2, a factor IX(a)-directed anticoagulant, inhibits arterial thrombosis without impairing haemostasis


Nitrophorin 2, a factor IX(a)-directed anticoagulant, inhibits arterial thrombosis without impairing haemostasis



Thrombosis and Haemostasis 104(6): 1116-1123



ISSN/ISBN: 0340-6245

PMID: 20838739

DOI: 10.1160/th10-03-0186

Nitrophorin 2 (NP2) is a 20 kDa lipocalin identified in the salivary gland of the blood sucking insect, Rhodnius prolixus. It functions as a potent inhibitor of the intrinsic pathway of coagulation upon binding to factor IX (FIX) or FIXa. Herein we have investigated the in vivo antithrombotic properties of NP2. Surface plasmon resonance assays demonstrated that NP2 binds to rat FIX and FIXa with high affinities (KD = 43 and 47 nM, respectively), and prolongs the aPTT without affecting the PT. In order to evaluate NP2 antithrombotic effects in vivo two distinct models of thrombosis in rats were carried out. In the rose Bengal/laser induced injury model of arterial thrombosis, NP2 increased the carotid artery occlusion time by ≍35 and ≍155%, at doses of 8 and 80 μg/kg, respectively. NP2 also inhibited thrombus formation in an arterio-venous shunt model, showing ≍60% reduction at 400 μg/kg (i.v. administration). The antithrombotic effect lasted for up to 48 hours after a single i.v. dose. Notably, effective doses of NP2 did not increase the blood loss as evaluated by tail-transection model. In conclusion, NP2 is a potent and long-lasting inhibitor of arterial thrombosis with minor effects on haemostasis. It might be regarded as a potential agent for the treatment of human cardiovascular diseases.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 054628381

Download citation: RISBibTeXText

Related references

Recombinant human annexin V inhibits intravascular arterial thrombosis without impairing extravascular hemostasis. Circulation 92(8 Suppl. ): I805, 1995

Monoclonal antibody to tissue factor inhibits intravascular thrombosis without impairing extravascular hemostasis. Journal of the American College of Cardiology 1995(SPEC ISSUE): 366A, 1995

Guidelines on testing for the lupus anticoagulant. Lupus Anticoagulant Working Party on behalf of the BCSH Haemostasis and Thrombosis Task Force. Journal of Clinical Pathology 44(11): 885-889, 1991

Update of the guidelines for lupus anticoagulant detection. Subcommittee on Lupus Anticoagulant/Antiphospholipid Antibody of the Scientific and Standardisation Committee of the International Society on Thrombosis and Haemostasis. Journal of thrombosis and haemostasis: JTH 7(10): 1737-1740, 2009

A human antibody that inhibits factor IX/IXa function potently inhibits arterial thrombosis without increasing bleeding. Arteriosclerosis Thrombosis and Vascular Biology 22(3): 517-522, 2002

Desmolaris, a novel factor XIa anticoagulant from the salivary gland of the vampire bat (Desmodus rotundus) inhibits inflammation and thrombosis in vivo. Blood 122(25): 4094-4106, 2013

Guidelines on the prevention, investigation and management of thrombosis associated with pregnancy. Maternal and Neonatal haemostasis Working Party of the Haemostasis and Thrombosis Task. Journal of Clinical Pathology 46(6): 489-496, 1993

Studies on the Site of Action of a Circulating Anticoagulant in Disseminated Lupus Erythematosus. Evidence that this Anticoagulant Inhibits the Reaction Between Activated Stuart Factor (Factor X) and Proaccelerin (Factor V). American journal of medicine 35: 813-819, 1963

Rheological aspects of thrombosis and haemostasis: basic principles and applications. ICTH-Report--Subcommittee on Rheology of the International Committee on Thrombosis and Haemostasis. Thrombosis and Haemostasis 55(3): 415-435, 1986

KLF11 (Krüppel-Like Factor 11) Inhibits Arterial Thrombosis via Suppression of Tissue Factor in the Vascular Wall. Arteriosclerosis Thrombosis and Vascular Biology 39(3): 402-412, 2019

Antithrombotic effect of the factor xa inhibitor tick anticoagulant peptide tap in a baboon model of acute arterial thrombosis. Circulation 82(4 Suppl. 3): III660, 1990

Progressive arterial thrombosis in a patient with non-Hodgkin's lymphoma, a lupus anticoagulant, factor V Leiden mutation and paraprotein, following chemotherapy. Leukemia and Lymphoma 42(1-2): 221-223, 2001

Antithrombotic efficacy of recombinant tick anticoagulant peptide. A potent inhibitor of coagulation factor Xa in a primate model of arterial thrombosis. Circulation 84(4): 1741-1748, 1991

Breaking the proximal Fe(II)-N(His) bond in heme proteins through local structural tension: lessons from the heme b proteins nitrophorin 4, nitrophorin 7, and related site-directed mutant proteins. Biochemistry 50(40): 8559-8575, 2011

Surgery and invasive procedures in patients on long-term treatment with direct oral anticoagulants: thrombin or factor-Xa inhibitors. Recommendations of the Working Group on Perioperative Haemostasis and the French Study Group on Thrombosis and Haemostasis. Archives of Cardiovascular Diseases 104(12): 669-676, 2011