+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Nodule inception directly targets NF-Y subunit genes to regulate essential processes of root nodule development in Lotus japonicus



Nodule inception directly targets NF-Y subunit genes to regulate essential processes of root nodule development in Lotus japonicus



Plos Genetics 9(3): E1003352



The interactions of legumes with symbiotic nitrogen-fixing bacteria cause the formation of specialized lateral root organs called root nodules. It has been postulated that this root nodule symbiosis system has recruited factors that act in early signaling pathways (common SYM genes) partly from the ancestral mycorrhizal symbiosis. However, the origins of factors needed for root nodule organogenesis are largely unknown. NODULE INCEPTION (NIN) is a nodulation-specific gene that encodes a putative transcription factor and acts downstream of the common SYM genes. Here, we identified two Nuclear Factor-Y (NF-Y) subunit genes, LjNF-YA1 and LjNF-YB1, as transcriptional targets of NIN in Lotus japonicus. These genes are expressed in root nodule primordia and their translational products interact in plant cells, indicating that they form an NF-Y complex in root nodule primordia. The knockdown of LjNF-YA1 inhibited root nodule organogenesis, as did the loss of function of NIN. Furthermore, we found that NIN overexpression induced root nodule primordium-like structures that originated from cortical cells in the absence of bacterial symbionts. Thus, NIN is a crucial factor responsible for initiating nodulation-specific symbiotic processes. In addition, ectopic expression of either NIN or the NF-Y subunit genes caused abnormal cell division during lateral root development. This indicated that the Lotus NF-Y subunits can function to stimulate cell division. Thus, transcriptional regulation by NIN, including the activation of the NF-Y subunit genes, induces cortical cell division, which is an initial step in root nodule organogenesis. Unlike the legume-specific NIN protein, NF-Y is a major CCAAT box binding protein complex that is widespread among eukaryotes. We propose that the evolution of root nodules in legume plants was associated with changes in the function of NIN. NIN has acquired functions that allow it to divert pathways involved in the regulation of cell division to root nodule organogenesis.

(PDF emailed within 0-6 h: $19.90)

Accession: 054634371

Download citation: RISBibTeXText

PMID: 23555278

DOI: 10.1371/journal.pgen.1003352


Related references

A Positive Regulator of Nodule Organogenesis, NODULE INCEPTION, Acts as a Negative Regulator of Rhizobial Infection in Lotus japonicus. Plant Physiology 165(2): 747-758, 2014

Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities. Proceedings of the National Academy of Sciences of the United States of America 113(49): E7996-E8005, 2016

Lotus japonicus NF-YA1 Plays an Essential Role During Nodule Differentiation and Targets Members of the SHI/STY Gene Family. Molecular Plant-Microbe Interactions 29(12): 950-964, 2016

Auxin distribution in Lotus japonicus during root nodule development. Plant molecular biology 52(6): 1169-1180, 2003

NODULE INCEPTION antagonistically regulates gene expression with nitrate in Lotus japonicus. Plant and Cell Physiology 56(2): 368-376, 2015

Involvement of auxin distribution in root nodule development of Lotus japonicus. Planta 234(1): 73-81, 2011

The Sym35 gene required for root nodule development in pea is an ortholog of Nin from Lotus japonicus. Plant Physiology 131(3): 1009-1017, 2003

A protein phosphatase 2C gene, LjNPP2C1, from Lotus japonicus induced during root nodule development. Proceedings of the National Academy of Sciences of the United States of America, 96(4): 1738-1743, 1999

Lotus japonicus NOOT-BOP-COCH-LIKE1 is essential for nodule, nectary, leaf and flower development. Plant Journal 94(5): 880-894, 2018

Common symbiosis genes CERBERUS and NSP1 provide additional insight into the establishment of arbuscular mycorrhizal and root nodule symbioses in Lotus japonicus. Plant Signaling and Behavior 9(3): E28544, 2015

Positive and negative regulation of cortical cell division during root nodule development in Lotus japonicus is accompanied by auxin response. Development 139(21): 3997-4006, 2012

The temperature-sensitive brush mutant of the legume Lotus japonicus reveals a link between root development and nodule infection by rhizobia. Plant Physiology 149(4): 1785-1796, 2009

TRICOT encodes an AMP1-related carboxypeptidase that regulates root nodule development and shoot apical meristem maintenance in Lotus japonicus. Development 140(2): 353-361, 2013

Proteome reference maps of the Lotus japonicus nodule and root. Proteomics 14(2-3): 230-240, 2014

CYTOKININ OXIDASE/DEHYDROGENASE3 Maintains Cytokinin Homeostasis during Root and Nodule Development in Lotus japonicus. Plant Physiology 170(2): 1060-1074, 2016