+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Novel Phenotypic Fluorescent Three-Dimensional Platforms for High-throughput Drug Screening and Personalized Chemotherapy



Novel Phenotypic Fluorescent Three-Dimensional Platforms for High-throughput Drug Screening and Personalized Chemotherapy



Journal of Cancer 4(5): 402-415



We have developed novel phenotypic fluorescent three-dimensional co-culture platforms that efficiently and economically screen anti-angiogenic/anti-metastatic drugs on a high-throughput scale. Individual cell populations can be identified and isolated for protein/gene expression profiling studies and cellular movement/interactions can be tracked by time-lapse cinematography. More importantly, these platforms closely parallel the in vivo angiogenic and metastatic outcomes of a given tumor xenograft in the nude mouse model but, unlike in vivo models, our co-culture platforms produce comparable results in five to nine days. Potentially, by incorporating cancer patient biopsies, the co-culture platforms should greatly improve the effectiveness and efficiency of personalized chemotherapy.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 054667555

Download citation: RISBibTeXText

PMID: 23833685

DOI: 10.7150/jca.6780


Related references

Novel Phenotypic Fluorescent Three-Dimensional Co-Culture Platforms for Recapitulating Tumor in vivo Progression and for Personalized Therapy. Journal of Cancer 4(9): 755-763, 2013

High-Content and High-Throughput In Vivo Drug Screening Platforms Using Microfluidics. Assay and Drug Development Technologies 17(1): 8-13, 2019

Integration of genomics, high throughput drug screening, and personalized xenograft models as a novel precision medicine paradigm for high risk pediatric cancer. Cancer Biology and Therapy 2018: 1-10, 2018

MyoScreen, a High-Throughput Phenotypic Screening Platform Enabling Muscle Drug Discovery. Slas Discovery 23(8): 790-806, 2018

High-Throughput Phenotypic Screening of Kinase Inhibitors to Identify Drug Targets for Polycystic Kidney Disease. Slas Discovery 22(8): 974-984, 2017

Advanced Development of Primary Pancreatic Organoid Tumor Models for High-Throughput Phenotypic Drug Screening. Slas Discovery 23(6): 574-584, 2018

A Fully Automated High-Throughput Flow Cytometry Screening System Enabling Phenotypic Drug Discovery. Slas Discovery 23(7): 697-707, 2018

Stably transformed insect cell lines: tools for expression of secreted and membrane-anchored proteins and high-throughput screening platforms for drug and insecticide discovery. Advances in Virus Research 68: 113-156, 2006

Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling. Nature Methods 3(5): 361-368, 2006

Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening. Journal of Visualized Experiments 2018(139), 2018

Drug Discovery Goes Three-Dimensional: Goodbye to Flat High-Throughput Screening?. Assay and Drug Development Technologies 13(5): 262-265, 2016

FRET-based calcium imaging: a tool for high-throughput/content phenotypic drug screening in Alzheimer disease. Journal of Biomolecular Screening 18(10): 1309-1320, 2014

Quantitative high-throughput phenotypic screening of pediatric cancer cell lines identifies multiple opportunities for drug repurposing. Oncotarget 9(4): 4758-4772, 2018

Pdtm-31. Identification Of Personalized Active Agents In Pediatric Gliomas Through High-Throughput Drug Screening In Matching Pairs Of Patient Derived Orthotopic Xenograft (Pdox) Neurosphere And Monolayer Cells. Neuro-Oncology 19(Suppl_6): vi196, 2017

Platforms for high-throughput screening of Wnt/Frizzled antagonists. Drug Discovery Today 17(23-24): 1316-1322, 2013