+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Nuclear myosin 1c facilitates the chromatin modifications required to activate rRNA gene transcription and cell cycle progression



Nuclear myosin 1c facilitates the chromatin modifications required to activate rRNA gene transcription and cell cycle progression



Plos Genetics 9(3): E1003397



Actin and nuclear myosin 1c (NM1) cooperate in RNA polymerase I (pol I) transcription. NM1 is also part of a multiprotein assembly, B-WICH, which is involved in transcription. This assembly contains the chromatin remodeling complex WICH with its subunits WSTF and SNF2h. We report here that NM1 binds SNF2h with enhanced affinity upon impairment of the actin-binding function. ChIP analysis revealed that NM1, SNF2h, and actin gene occupancies are cell cycle-dependent and require intact motor function. At the onset of cell division, when transcription is temporarily blocked, B-WICH is disassembled due to WSTF phosphorylation, to be reassembled on the active gene at exit from mitosis. NM1 gene knockdown and motor function inhibition, or stable expression of NM1 mutants that do not interact with actin or chromatin, overall repressed rRNA synthesis by stalling pol I at the gene promoter, led to chromatin alterations by changing the state of H3K9 acetylation at gene promoter, and delayed cell cycle progression. These results suggest a unique structural role for NM1 in which the interaction with SNF2h stabilizes B-WICH at the gene promoter and facilitates recruitment of the HAT PCAF. This leads to a permissive chromatin structure required for transcription activation.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 054681916

Download citation: RISBibTeXText

PMID: 23555303

DOI: 10.1371/journal.pgen.1003397


Related references

The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression. Rna Biology 12(3): 255-267, 2015

The role and mechanism of progesterone receptor activation of extra-nuclear signaling pathways in regulating gene transcription and cell cycle progression. Steroids 73(9-10): 922-928, 2008

Modifications of nuclear architecture and chromatin organization in ataxia telangiectasia cells are coupled to changes of gene transcription. Journal of Cellular Biochemistry 99(4): 1148-1164, 2006

Loss of nuclear pro-IL-16 facilitates cell cycle progression in human cutaneous T cell lymphoma. Journal of Clinical Investigation 121(12): 4838-4849, 2012

Retraction: Loss of nuclear pro-IL-16 facilitates cell cycle progression in human cutaneous T cell lymphoma. Journal of Clinical Investigation 124(11): 5085-5085, 2015

Sfh1p, a component of a novel chromatin-remodeling complex, is required for cell cycle progression. Molecular and Cellular Biology 17(6): 3323-3334, 1997

Cell cycle regulation of Greatwall kinase nuclear localization facilitates mitotic progression. Journal of Cell Biology 202(2): 277-293, 2013

The Ets transcription factor GABP is required for cell-cycle progression. Nature Cell Biology 9(3): 339, 2007

Nuclear actin is involved in the regulation of CSF1 gene transcription in a chromatin required, BRG1 independent manner. Journal of Cellular Biochemistry 102(2): 403-411, 2007

Smads orchestrate specific histone modifications and chromatin remodeling to activate transcription. EMBO (European Molecular Biology Organization) Journal 25(19): 4490-4502, 2006

Yeast TAF(II)90 is required for cell-cycle progression through G2/M but not for general transcription activation. Genes & Development 10(18): 2368-2380, 1996

A gene function required for cell cycle progression during the G1 portion of the cell cycle and for maintenance of macronuclear DNA synthesis in Paramecium tetraurelia. Experimental Cell Research 155(2): 593-597, 1984

Polyomavirus small T antigen controls viral chromatin modifications through effects on kinetics of virus growth and cell cycle progression. Journal of Virology 81(18): 10064-10071, 2007

Nuclear RNA species activate DNA transcription within chromatin. FASEB Journal 13(7): A1506, April 23, 1999

Cyclin D1 is a nuclear protein required for cell cycle progression in G1. Genes and Development 7(5): 812-821, 1993