+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Optimization of an Adsorption-Elution Method with a Negatively Charged Membrane to Recover Norovirus from Lettuce

Optimization of an Adsorption-Elution Method with a Negatively Charged Membrane to Recover Norovirus from Lettuce

Food and Environmental Virology 2013:

Viral pathogens, such as norovirus (NoV), are frequently associated with foodborne gastroenteritis worldwide, and the detection of NoV in food requires appropriate methods and the use of process controls. In this study, an adsorption-elution concentration method using negatively charged membranes was optimized to recover NoV from lettuce, using murine norovirus 1 (MNV-1) as a human NoV (HuNoV) surrogate. Initially, three elution buffers were evaluated by direct elution using a Stomacher® apparatus with a filter bag and different concentrations of MNV-1 genomic copies. The eluates were filtered in a Stericup® and concentrated by a Centriprep Concentrator®, and the viral RNA was quantified by real-time PCR that was preceded by reverse transcription. The MNV-1 recovery efficiency varied based on the buffers used, ranging from 5.2 to 9.8 % for PBS pH 7.2, 0.2-18 % for glycine NaCl pH 9.5 and 10.8-33.3 % for glycine Tris-HCl pH 9.5. Further analysis of the glycine Tris-HCl pH 9.5 buffer revealed that gentle-shaking, direct elution could replace the use of a Stomacher®, with recovery rates reaching 66 and 32 % for MNV-1 and HuNoV, respectively, all of which suggested that this procedure is a quick and efficient method for recovering NoV from lettuce.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 054772554

Download citation: RISBibTeXText

PMID: 23649411

DOI: 10.1007/s12560-013-9113-5

Related references

Adsorption-elution with negatively and positively-charged glass powder for the concentration of hepatitis A virus from water. Journal of Virological Methods 31(2-3): 345-351, 1991

How Negatively Charged Proteins Adsorb to Negatively Charged Surfaces: A Molecular Dynamics Study of BSA Adsorption on Silica. Journal of Physical Chemistry. B 120(40): 10463-10468, 2016

Evaluation of an adsorption-elution method for detection of astrovirus and norovirus in environmental waters. Journal of Virological Methods 156(1-2): 73-76, 2009

Recovery rate of multiple enteric viruses artificially seeded in water and concentrated by adsorption-elution with negatively charged membranes: interaction and interference between different virus species. Water Science and Technology 72(12): 2291-2300, 2015

Enhanced adsorption of Ca-ATPase containing vesicles on a negatively charged solid-supported-membrane for the investigation of membrane transporters. Langmuir 29(45): 13883-9, 2013

Determination of naturally occurring noroviruses in coastal seawater by alkaline elution after acid rinse using negatively charged membrane. Water Science and Technology: Water Supply 4(2): 73-77, 2004

Influence of architecture on the interaction of negatively charged multisensitive poly(N-isopropylacrylamide)-co-methacrylic acid microgels with oppositely charged polyelectrolyte: absorption vs adsorption. Langmuir 26(13): 11258-11265, 2010

Study on membrane adsorption-elution method for concentration of enteroviruses from environmental waters. Huan Jing Ke Xue= Huanjing Kexue 28(7): 1543-1547, 2007

Interaction between equally charged membrane surfaces mediated by positively and negatively charged macro-ions. Journal of Membrane Biology 236(1): 43-53, 2010

Differential inactivation of three bacteriophages by acid and alkaline pH used in the membrane adsorption-elution method of virus recovery. Canadian Journal of Microbiology 26(12): 1403-1407, 1980

The adsorption of human plasma albumin and bovine pancreas rnase at negatively charged poly styrene surfaces part 1 adsorption isotherms effects of charge ionic strength and temperature. Journal of Colloid and Interface Science 66(2): 257-265, 1978

On the growth-stunting effect of negatively charged air-ionization on livestock: A contribution to the problem of the germ-inhibiting action of negatively-charged air ions. 1972

Adsorption of horseradish peroxidase to negatively charged groups. Acta Histochemica 43(1): 8, 1972

The adsorption of F(ab')2 on positively and negatively charged polystyrene beads. Journal of Biomaterials Science. Polymer Edition 6(3): 269-279, 1994

Domain-specific adsorption of cytochrome c on negatively charged surfaces. Abstracts of Papers American Chemical Society 223(1-2): ANYL 13, 2002