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Overcoming glucose repression in mixed sugar fermentation by co-expressing a cellobiose transporter and a β-glucosidase in Saccharomyces cerevisiae

Overcoming glucose repression in mixed sugar fermentation by co-expressing a cellobiose transporter and a β-glucosidase in Saccharomyces cerevisiae

Molecular Biosystems 6(11): 2129-2132

Glucose repression is one of the main limitations in mixed lignocellulosic sugar fermentation for cost-effective production of fuels and chemicals. Here we report a novel strategy to overcome glucose repression by co-expressing a cellobiose transporter and a β-glucosidase in an engineered d-xylose-utilizing Saccharomyces cerevisiae strain. The resulting strain can simultaneously utilize cellobiose and d-xylose for ethanol production.

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Accession: 054817538

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PMID: 20871937

DOI: 10.1039/c0mb00063a

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