+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Overcoming glucose repression in mixed sugar fermentation by co-expressing a cellobiose transporter and a β-glucosidase in Saccharomyces cerevisiae



Overcoming glucose repression in mixed sugar fermentation by co-expressing a cellobiose transporter and a β-glucosidase in Saccharomyces cerevisiae



Molecular Biosystems 6(11): 2129-2132



Glucose repression is one of the main limitations in mixed lignocellulosic sugar fermentation for cost-effective production of fuels and chemicals. Here we report a novel strategy to overcome glucose repression by co-expressing a cellobiose transporter and a β-glucosidase in an engineered d-xylose-utilizing Saccharomyces cerevisiae strain. The resulting strain can simultaneously utilize cellobiose and d-xylose for ethanol production.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 054817538

Download citation: RISBibTeXText

PMID: 20871937

DOI: 10.1039/c0mb00063a


Related references

Improved ethanol production by engineered Saccharomyces cerevisiae expressing a mutated cellobiose transporter during simultaneous saccharification and fermentation. Journal of Biotechnology 245: 1-8, 2017

Enhanced cellobiose fermentation by engineered Saccharomyces cerevisiae expressing a mutant cellodextrin facilitator and cellobiose phosphorylase. Journal of Biotechnology 275: 53-59, 2018

Energetic benefits and rapid cellobiose fermentation by Saccharomyces cerevisiae expressing cellobiose phosphorylase and mutant cellodextrin transporters. Metabolic Engineering 15: 134-143, 2013

Studies on the ethanol fermentation of cellobiose by coimmobilized Saccharomyces cerevisiae and beta-glucosidase. Journal of the Chinese Agricultural Chemical Society 35(4): 433-444, 1997

Utilization of cellobiose by recombinant β-glucosidase-expressing strains of Saccharomyces cerevisiae: characterization and evaluation of the sufficiency of expression. Enzyme and Microbial Technology 37(1): 93-101, 2005

Induction and repression of extra cellular beta glucosidase in brettanomyces clausenii fermentation on glucose and cellobiose. Abstracts of Papers American Chemical Society 194: MBTD 137, 1987

Transporter engineering for cellobiose fermentation under lower pH conditions by engineered Saccharomyces cerevisiae. Bioresource Technology 245(Pt B): 1469-1475, 2017

Co-fermentation of cellobiose and xylose by mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling. Plos one 13(6): E0199104, 2018

Deletion of the HXK2 gene in Saccharomyces cerevisiae enables mixed sugar fermentation of glucose and galactose in oxygen-limited conditions. Process Biochemistry 49(4): 547-553, 2014

Growth and glucose repression are controlled by glucose transport in Saccharomyces cerevisiae cells containing only one glucose transporter. Journal of Bacteriology 181(15): 4673-4675, 1999

Simultaneous saccharification and fermentation of acid-pretreated corncobs with a recombinant Saccharomyces cerevisiae expressing beta-glucosidase. Bioresource Technology 99(11): 5099-5103, 2008

Molecular cloning and expression of fungal cellobiose transporters and β-glucosidases conferring efficient cellobiose fermentation in Saccharomyces cerevisiae. Journal of Biotechnology 169: 34-41, 2014

Kinetic analysis of glucose transport in wild-type and transporter-deficient Saccharomyces cerevisiae strains under glucose repression and derepression. Journal Of Biotechnology. 27(1): 47-57, 1992

Enz alpha glucosidase synthesis respiratory enzymes and catabolite repression in yeast iv a studies on the level of protein synthesis at which repression ind induction of enz alpha glucosidase synthesis occur saccharomyces carlsbergensis saccharomyces cerevisiae. Proceedings of the Koninklijke Nederlandse Akademie van Wetenschappen Series C Biological & Medical Sciences 71(3): 293-301, 1968

Trehalose-6-phosphate promotes fermentation and glucose repression in Saccharomyces cerevisiae. Microbial Cell 5(10): 444-459, 2018