+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Overexpression, purification, and biochemical characterization of the esterase Est0796 from Lactobacillus plantarum WCFS1



Overexpression, purification, and biochemical characterization of the esterase Est0796 from Lactobacillus plantarum WCFS1



Molecular Biotechnology 54(2): 651-660



Lactobacillus plantarum is a lactic acid bacterium that is encountered in an extensive range of foods such as fermented dairy products, meat, vegetables, and bakery products. Given the little molecular information available on the lipolytic activity of L. plantarum, the aim of this study was to clone, purify, and biochemically characterize the esterase coded by gene lp_0796 (Est0796). The esterase was cloned in pET28a and purified in two steps, using solid ammonium sulfate and His tag affinity chromatography. The molecular mass of the purified Est0796 was 28.7 kDa (by SDS-PAGE) and 26.6 kDa (by gel filtration chromatography), pointing to a monomeric structure. Est0796 showed maximum activity at pH 8.0 and 35 °C and toward shorter acyl chain lengths (C2-C4). The activity was resistant to organic solvents and cations, suggesting that this esterase may play a role in the fermentation of food products.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 054820685

Download citation: RISBibTeXText

PMID: 23054632

DOI: 10.1007/s12033-012-9607-7


Related references

Overexpression, Purification, and Biochemical Characterization of the Esterase Est796 from Lactobacillus plantarum WCFS. 2012

Structure, biochemical characterization and analysis of the pleomorphism of carboxylesterase Cest-2923 from Lactobacillus plantarum WCFS1. Febs Journal 280(24): 6658-6671, 2013

Beta-Galactosidase from Lactobacillus plantarum WCFS1: biochemical characterization and formation of prebiotic galacto-oligosaccharides. Carbohydrate Research 345(10): 1408-1416, 2010

Preliminary X-ray analysis of twinned crystals of the Q88Y25_Lacpl esterase from Lactobacillus plantarum WCFS1. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 67(Pt 11): 1436-1439, 2011

Cloning, expression, purification, and characterization of a novel esterase from Lactobacillus plantarum. Molecular Biotechnology 44(3): 242-249, 2010

Chitinase from Bacillus licheniformis DSM13: expression in Lactobacillus plantarum WCFS1 and biochemical characterisation. Protein Expression and Purification 81(2): 166-174, 2012

Molecular characterization of a novel N-acetylneuraminate lyase from Lactobacillus plantarum WCFS1. Applied and Environmental Microbiology 77(7): 2471-2478, 2011

Characterization of a benzyl alcohol dehydrogenase from Lactobacillus plantarum WCFS1. Journal of Agricultural and Food Chemistry 56(12): 4497-4503, 2008

Purification and biochemical characterization of pyruvate oxidase from Lactobacillus plantarum. Journal of Bacteriology 160(1): 273-278, 1984

Molecular characterization of an inducible p-coumaric acid decarboxylase from Lactobacillus plantarum: gene cloning, transcriptional analysis, overexpression in Escherichia coli, purification, and characterization. Applied and Environmental Microbiology 63(5): 1939-1944, 1997

Characterization of a nitroreductase with selective nitroreduction properties in the food and intestinal lactic acid bacterium Lactobacillus plantarum WCFS1. Journal of Agricultural and Food Chemistry 57(21): 10457-10465, 2009

Characterization of two putative prolinases (PepR1 and PepR2) from Lactobacillus plantarum WCFS1: occurrence of two isozymes with structural similarity and different catalytic properties. Biochimica et Biophysica Acta 1854(2): 91, 2015

Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime. Food and Function 7(6): 2840-2848, 2016

Distinct immunomodulation of bone marrow-derived dendritic cell responses to Lactobacillus plantarum WCFS1 by two different polysaccharides isolated from Lactobacillus rhamnosus LOCK 0900. Applied and Environmental Microbiology 80(20): 6506-6516, 2014

Lactobacillus rhamnosus LA68 and Lactobacillus plantarum WCFS1 differently influence metabolic and immunological parameters in high fat diet-induced hypercholesterolemia and hepatic steatosis. Food and Function 6(2): 558-565, 2015