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PNA-based fluorescence in situ hybridization for identification of bacteria in clinical samples


PNA-based fluorescence in situ hybridization for identification of bacteria in clinical samples



Methods in Molecular Biology 1211: 261-271



ISSN/ISBN: 1064-3745

PMID: 25218392

DOI: 10.1007/978-1-4939-1459-3_21

Fluorescence in situ hybridization with PNA probes (PNA-FISH) that target specific bacterial ribosomal RNA sequences is a powerful and rapid tool for identification of bacteria in clinical samples. PNA can diffuse readily through the bacterial cell wall due to its uncharged backbone, and PNA-FISH can be performed with high specificity due to the extraordinary thermal stability of RNA-PNA hybrid complexes. We describe a PNA-FISH procedure and provide examples of the application of PNA-FISH for the identification of bacteria in chronic wounds, cystic fibrosis lungs, and soft tissue fillers. In all these cases, bacteria can be identified in biofilm aggregates, which may explain their recalcitrance to antibiotic treatment.

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Accession: 054839454

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