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Parallelized STED fluorescence nanoscopy

Parallelized STED fluorescence nanoscopy

Optics Express 19(24): 23716-23726

We introduce a parallelized STED microscope featuring m = 4 pairs of scanning excitation and STED beams, providing m-fold increased imaging speed of a given sample area, while maintaining basically all of the advantages of single beam scanning. Requiring only a single laser source and fiber input, the setup is inherently aligned both spatially and temporally. Given enough laser power, the design is readily scalable to higher degrees of parallelization m.

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Accession: 054860944

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PMID: 22109398

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