+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Polymorphisms of inhibin α gene exon 1 in buffalo (Bubalus bubalis), gayal (Bos frontalis) and yak (Bos grunniens)



Polymorphisms of inhibin α gene exon 1 in buffalo (Bubalus bubalis), gayal (Bos frontalis) and yak (Bos grunniens)



Dong Wu Xue Yan Jiu 33(4): 402-408



To elucidate the genetic characteristics of the bovine Inhibin α subunit (INHA) gene, the polymorphisms in exon 1 of INHA and its bilateral sequences were assayed using PCR with direct sequencing in buffalo, gayal and yak. A comparative analysis was conducted by pooled the results in this study with the published data of INHA on some mammals including some bovine species together. A synonymous substitution c.73C>A was identified in exon 1 of INHA for buffalo, which results in identical encoding product in river and swamp buffalo. In gayal, two non-synonymous but same property substitutions in exon 1 of INHA, viz. c.62 C>T and c.187 G>A, were detected, which lead to p. P21L, p. V63M changes in INHA, respectively. In yak, nucleotide substitution c.62C> T, c.129A>G were found in exon 1 of INHA, the former still causes p. P21L substitution and the latter is synonymous. For the sequence of the 5'-flanking region of INHA examined, no SNPs were found within the species, but a substitution, c. -6T>G, was found. The nucleotide in this site in gayal, yak and cattle was c. -6G, whereas in buffalo it was c. -6T. Meanwhile, a 6-bp deletion, namely c. 262+31_262+36delTCTGAC, was found in the intron of buffalo INHA gene. For this deletion, wild types (+/+) account for main part in river buffalo while mutant types (-/-) are predominant in swamp buffalo. This deletion was not found in gayal, yak and cattle, though these all have another deletion in the intron of INHA, c. 262+78_262+79delTG. The results of sequence alignment showed that the substitutions c. 43A and c. 67G in exon 1 of INHA are specific to buffalo, whereas the substitutions c. 173A and c. 255G are exclusive to gayal, yak and cattle, and c. 24C, c. 47G, c. 174T and c. 206T are specific to goat. Furthermore, there are few differences among gayal, yak and cattle, but there relatively great differences between buffalo, goat and other bovine species regarding the sequences of INHA exon 1.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 055047955

Download citation: RISBibTeXText

PMID: 22855448


Related references

Genotypic analysis of exon IV of the buffalo (Bubalus bubalis) kappa -casein gene. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 50(4): 415-419, 1998

Association of a novel SNP in exon 10 of the IGF2 gene with growth traits in Egyptian water buffalo (Bubalus bubalis). Tropical Animal Health and Production 46(6): 947-952, 2014

Polymorphism in exon 2 of the BuLA-DRB3 gene in Indian buffalo (Bubalus bubalis var. indicus) detected by PCR-RFLP. Animal Science 70(2): 221-226, 2000

Seven novel single nucleotide polymorphisms identified within river buffalo (Bubalus bubalis) lactoferrin gene. Tropical Animal Health and Production 42(5): 1021-1026, 2010

Postpartum endocrinology and prospects for fertility improvement in the lactating riverine buffalo (Bubalus bubalis) and yak (Poephagus grunniens L.). Livestock Production Science 98(1/2): 13-23, 2005

Novel polymorphisms of the IGF1R gene and their association with average daily gain in Egyptian buffalo (Bubalus bubalis). Domestic Animal Endocrinology 45(2): 105-110, 2013

Detection of prion gene promoter and intron1 indel polymorphisms in Anatolian water buffalo (Bubalus bubalis). Journal of Animal Breeding and Genetics 126(6): 463-467, 2009

Peripheral inhibin levels during estrous cycle in buffalo (Bubalus bubalis). Theriogenology 45(3): 655-664, 1996

Presence of inhibin in testes of the Indian water buffalo Bubalus bubalis. Israel Journal of Medical Sciences 17(8): 697-702, 1981

Multiple molecular forms of inhibin in buffalo (Bubalus bubalis) ovarian follicular fluid. Research in Veterinary Science 89(1): 14-19, 2010

MHC-DRB exon 2 allele polymorphism in Indian river buffalo (Bubalus bubalis). Animal Genetics 33(3): 215-219, 2002

Peripheral inhibin levels in relation to climatic variations and stage of estrous cycle in buffalo (Bubalus bubalis). Theriogenology 47(5): 989-995, 1997

Characteristics of gametogenesis of hybrids of the Tibetan yak, Bos (Poephagus) grunniens L., with the gayal, Bos (Bibos) frontalis Lambert. Tsitol. Genet 3: 274-279, 1969

A comparative study on mimosine, 3,4-dihydroxy pyridone (3,4-DHP) and 2,3-dihydroxy pyridone (2,3-DHP), purine derivatives (PD) excretion in the urine, thyroid hormone and blood metabolites profiles of Thai swamp buffalo (Bubalus bubalis) and Murrah buffalo (Bubalus bubalis). Tropical Animal Health and Production 44(4): 887-897, 2012

Novel polymorphisms in UTR and coding region of inducible heat shock protein 70.1 gene in tropically adapted Indian zebu cattle (Bos indicus) and riverine buffalo (Bubalus bubalis). Gene 527(2): 606-615, 2013