+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Pre-induced adult human peripheral blood mononuclear cells migrate widely into the degenerative retinas of rd1 mice



Pre-induced adult human peripheral blood mononuclear cells migrate widely into the degenerative retinas of rd1 mice



CytoTherapy 15(11): 1416-1425



Recent advances in stem cell research have raised the possibility of stem cells repairing or replacing retinal photoreceptor cells that are either dysfunctional or lost in many retinal diseases. Various types of stem cells have been used to replace retinal photoreceptor cells. Recently, peripheral blood stem cells, a small proportion of pluripotent stem cells, have been reported to mainly exist in the peripheral blood mononuclear cells (PBMCs). In this study, the effects of pre-induced adult human PBMCs (hPBMCs) on the degenerative retinas of rd1 mice were investigated. Freshly isolated adult hPBMCs were pre-induced with the use of the conditioned medium of rat retinas for 4 days and were then labeled with chloromethyl-benzamidodialkylcarbocyanine (CM-DiI) and then transplanted into the subretinal space of the right eye of rd1 mice through a trans-scleral approach. The right eyes were collected 30 days after transplantation. The survival and migration of the transplanted cells in host retinas were investigated by whole-mount retinas, retinal frozen sections and immunofluorescent staining. After subretinal transplantation, pre-induced hPBMCs were able to survive and widely migrate into the retinas of rd1 mice. A few CM-DiI-labeled cells migrated into the inner nuclear layer and the retinal ganglion cell layer. Some transplanted cells in the subretinal space of rd1 host mice expressed the human photoreceptor-specific marker rhodopsin. This study suggests that pre-induced hPBMCs may be a potential cell source of cell replacement therapy for retinal degenerative diseases.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 055092250

Download citation: RISBibTeXText

PMID: 24094491

DOI: 10.1016/j.jcyt.2013.05.025


Related references

Xenogeneic graft-versus-host disease in neonatal SCID mice transplanted with adult human peripheral blood mononuclear cells but not cord blood mononuclear cells. FASEB Journal 8(4-5): A754, 1994

Survival and migration of pre-induced adult human peripheral blood mononuclear cells in retinal degeneration slow (rds) mice three months after subretinal transplantation. Current Stem Cell Research and Therapy 9(2): 124-133, 2014

Survival and Migration of Pre-induced Adult Human Peripheral Blood Mononuclear Cells in Retinal Degeneration Slow (rds) Mice Three Months After Subretinal Transplantation. Current Stem Cell Research & Therapy 9(2): 124-133, 2014

Recombinant interleukin 2 inhibits pokeweed mitogen-induced proliferation of human adult peripheral blood and cord blood mononuclear cells. Immunology Letters 18(3): 231-236, 1988

Peripheral blood mononuclear cells from recipients of human bone marrow allografts suppress mitogen induced proliferation of marrow donor peripheral blood mononuclear cells. Transplantation Proceedings: 270-272, 1986

Conversion of adult human peripheral blood mononuclear cells into induced neural stem cell by using episomal vectors. Stem Cell Research 16(2): 236-242, 2016

CD14+ CD34+ peripheral blood mononuclear cells migrate across endothelium and give rise to immunostimulatory dendritic cells. Journal of Immunology 160(6): 2675-2683, 1998

Synthesis and secretion of IgA, IgM, and IgG by peripheral blood mononuclear cells in human disease states, by isolated human intestinal mononuclear cells, and by human bone marrow mononuclear cells from ribs. Annals of the New York Academy of Sciences 409: 498-509, 1983

Correlations between enzymatic and immunologic properties of human peripheral blood mononuclear cells 1. ecto enzymes of normal and immuno deficient peripheral blood mononuclear cells. American Journal of Pathology 107(2): 191-201, 1982

gamma-Interferon production in peripheral blood mononuclear cells and tumor infiltrating lymphocytes from Kaposi's sarcoma patients: correlation with the presence of human herpesvirus-8 in peripheral blood mononuclear cells and lesional macrophages. Blood 91(3): 968-976, 1998

Characterization of Epstein-Barr virus-induced lymphoproliferation derived from human peripheral blood mononuclear cells transferred to severe combined immunodeficient mice. American Journal of Pathology 137(3): 517-522, 1990

Mechanistic studies of the lysis of human umbilical vein endothelial cells by cytokine-induced killer cells expanded from peripheral blood mononuclear cells and cord blood. Blood 88(10 Suppl. 1 Part 1-2): 39B, 1996

Fasting induces changes in peripheral blood mononuclear cell gene expression profiles related to increases in fatty acid -oxidation: functional role of peroxisome proliferatoractivated receptor in human peripheral blood mononuclear cells. The American Journal of Clinical Nutrition 86(5): 1515-1523, 2007

Fasting induces changes in peripheral blood mononuclear cell gene expression profiles related to increases in fatty acid o-oxidation: functional role of peroxisome proliferator-activated receptor l in human peripheral blood mononuclear cells. American journal of clinical nutrition AJN 86(5): 1515-1523, 2007

Lack of activation induced cell death distinguishes oral blood mononuclear cells from peripheral blood mononuclear cells. FASEB Journal 18(4-5): Abst 88 16, 2004