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Preparation and characterization of the epitopes of SARS coronavirus spike protein



Preparation and characterization of the epitopes of SARS coronavirus spike protein



Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 23(11): 1007-1009



To prepare the recombinant epitopes of SARS-CoV spike protein and study their antigenic property to spike protein. The epitopes of SARS-CoV spike protein were analyzed by epitope prediction software. A novel gene, named Z, encoding 16 predicted epitopes of spike protein was designed and synthesized using chemical method. Z gene was cloned into vector pET-28a(+), expressed in E.coli BL21(DE3) and purified by Ni(2+) affinity method. Z protein was used as antigen to immunize the rabbit and anti-Z sera was collected. Then the antigenic property of Z protein to SARS-CoV spike protein was analyzed by dot-blot and ELISA assay. Z gene was successfully designed and expressed in E.coli BL21(DE3). Dot blot analysis showed that SARS-CoV spike protein, which was expressed and purified from mammal cells, can be detected by anti-Z sera from rabbit. ELISA analysis indicated the SARS-CoV antigen prepared from SARS-CoV lysates can be detected by anti-Z sera. The predicted epitopes of Z protein can induce the development of SARS-CoV spike protein antibody in rabbits, which provides a new protein for developing vaccine against SARS-CoV.

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Accession: 055132069

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PMID: 17988578


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