+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Preparation and evaluation of a molecularly imprinted polymer for the selective recognition of testosterone--application to molecularly imprinted sorbent assays



Preparation and evaluation of a molecularly imprinted polymer for the selective recognition of testosterone--application to molecularly imprinted sorbent assays



Journal of Molecular Recognition 24(6): 1123-1129



Biomimetic testosterone receptors were synthesized via molecular imprinting for use as antibody mimics in immunoassays. As evaluated by radioligand binding assays, imprinted polymers prepared in acetonitrile were very specific for testosterone because the nonimprinted control polymers bound virtually no radiolabeled testosterone. The polymers present an appreciable affinity, with association constants of K(a) = 3.3 x 10(7) M(- 1) (high-affinity binding sites). The binding characteristics of the polymers were also evaluated in aqueous environment to study their viabilities as alternatives to antibodies in molecularly imprinted sorbent assays. Compared with the testosterone-specific antibodies present in commercial kits, our molecularly imprinted polymers are somewhat less sensitive but show a high selectivity.

(PDF emailed within 0-6 h: $19.90)

Accession: 055132338

Download citation: RISBibTeXText

PMID: 22038819

DOI: 10.1002/jmr.1162


Related references

Preparation, Characterization and Application of a Molecularly Imprinted Polymer for Selective Recognition of Sulpiride. Materials 10(5), 2017

Preparation and evaluation of molecularly imprinted polymer for selective recognition and adsorption of gossypol. Journal of Molecular Recognition 31(3), 2017

Preparation, evaluation and application of diazinon imprinted polymers as the sorbent in molecularly imprinted solid-phase extraction and liquid chromatography analysis in cucumber and aqueous samples. Food Chemistry 158: 421-428, 2015

Highly sensitive and selective hyphenated technique (molecularly imprinted polymer solid-phase microextraction-molecularly imprinted polymer sensor) for ultra trace analysis of aspartic acid enantiomers. Journal of Chromatography. a 1283: 9-19, 2013

Highly selective and sensitive analysis of dopamine by molecularly imprinted stir bar sorptive extraction technique coupled with complementary molecularly imprinted polymer sensor. Journal of Colloid and Interface Science 396: 234-241, 2013

Molecularly imprinted uniform-sized polymer-based stationary phase for naproxen. Comparison of molecular recognition ability of the molecularly imprinted polymers prepared by thermal and redox polymerization techniques. Journal of Chromatography A 816(2): 113-121, 1998

Preparation and characterization of a molecularly imprinted polymer by grafting on silica supports: a selective sorbent for patulin toxin. Analytical and Bioanalytical Chemistry 401(7): 2259-2273, 2012

Molecularly imprinted polymer film grafted from porous silica for selective recognition of testosterone. Analytical Letters 39(2): 275-286, 2006

Recognition properties and competitive assays of a dual dopamine/serotonin selective molecularly imprinted polymer. International Journal of Molecular Sciences 9(12): 2333-2356, 2009

Preparation of magnetic molecularly imprinted polymer for selective recognition of resveratrol in wine. Journal of Chromatography. a 1300: 112-118, 2014

Development of a highly sensitive and selective hyphenated technique (molecularly imprinted micro-solid phase extraction fiber-molecularly imprinted polymer fiber sensor) for ultratrace analysis of folic acid. Analytica Chimica Acta 662(1): 14-22, 2010

Zwitterionic molecularly imprinted polymer-based solid-phase micro-extraction coupled with molecularly imprinted polymer sensor for ultra-trace sensing of L-histidine. Journal of Separation Science 32(7): 1096-1105, 2009

Preparation of a hollow porous molecularly imprinted polymer using tetrabromobisphenol A as a dummy template and its application as SPE sorbent for determination of bisphenol A in tap water. Talanta 117: 281-287, 2014

Ultratrace analysis of uracil and 5-fluorouracil by molecularly imprinted polymer brushes grafted to silylated solid-phase microextraction fiber in combination with complementary molecularly imprinted polymer-based sensor. Biomedical Chromatography 23(5): 499-509, 2009

Molecularly imprinted solid-phase extraction combined with molecularly imprinted polymer-sensor: a diagnostic tool applicable to creatine deficiency syndrome. Biomedical Chromatography 21(9): 976-986, 2007