Preparation and in vitro characterization of dexamethasone-loaded poly (D,L-lactic acid) microspheres embedded in poly (ethylene glycol) -poly ({varepsilon}-caprolactone) -poly (ethylene glycol) hydrogel for orthopedic tissue engineering

Fan, M.; Guo, Q.; Luo, J.; Luo, F.; Xie, P.; Tang, X.; Qian, Z.

Journal of Biomaterials Applications 28(2): 288-297


ISSN/ISBN: 0885-3282
PMID: 22561978
DOI: 10.1177/0885328212446097
Accession: 055132759

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The corium is decreased to about half of its thickness in skin defects and wrinkles due to gravity and environment. In this study, dexamethasone/poly(d,l-lactic acid) (Mn = 160,000) microspheres were incorporated into poly(ethylene glycol)-poly(ε-caprolactone)-poly(ethylene glycol) (Mn = 3300) hydrogel to prepare an injectable hydrogel composite. The composite was designed to increase the thickness of the corium. Dexamethasone/poly(d,l-lactic acid) microspheres were prepared by oil-in-water emulsion/solvent evaporation technique. The properties of microspheres were investigated by size distribution measurement, scanning electron microscope and x-ray diffraction. Drug loading, encapsulation efficiency, and drug delivery behavior of microspheres were also studied in detail. Cell adhesion of microspheres was investigated by NIH3T3 cell in vitro. The properties of hydrogel composite were investigated by scanning electron microscope, rheological measurements and methyl thiazolyl tetrazolium assay. Drug release from composite was determined by HPLC-UV analysis. These results suggested that poly(d,l-lactic acid) microspheres encapsulating dexamethasone embedded in poly(ethylene glycol)-poly(ε-caprolactone)-poly(ethylene glycol) hydrogel might have prospective application in orthopedic tissue engineering field.