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Preparation of HCV NS3 and NS5B proteins to support small-molecule drug discovery



Preparation of HCV NS3 and NS5B proteins to support small-molecule drug discovery



Current Protocols in Pharmacology Chapter 13: Unit13b.6



Production of high-quality, well-characterized recombinant proteins facilitates screening of compound libraries. The protocols detailed in this unit are used to purify three recombinant enzymes that are widely used in HCV research: the HCV NS3 protease domain, the helicase domain as an NS3+NS4A complex, and the NS5B RNA-dependent RNA polymerase. The active enzymes are purified to homogeneity by two-column chromatography to support a screening program for HCV inhibitors.

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Accession: 055134023

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PMID: 21898331

DOI: 10.1002/0471141755.ph13b06s54


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