+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Probing the modes of antibacterial activity of chitosan. Effects of pH and molecular weight on chitosan interactions with membrane lipids in Langmuir films



Probing the modes of antibacterial activity of chitosan. Effects of pH and molecular weight on chitosan interactions with membrane lipids in Langmuir films



Biomacromolecules 12(11): 4144-4152



Chitosan, a cationic biopolymer derived from chitin, has been described as having antibacterial activity. The modes of this activity, however, have not been established. One mode proposed is that chitosan perturbs bacterial cell membranes. To validate this proposal, in this study we investigated chitosan interactions with lipids in Langmuir monolayers as model membranes. The interactions were assessed by monitoring differences in the shape of the compression isotherms measured in the absence and presence of chitosan in the subphase (acetate buffer). To appraise the contribution of electrostatic interactions versus hydrogen bonding and hydrophobic interactions, three membrane lipids differing in charge were studied-anionic dipalmitoylphosphatidylglycerol (DPPG), zwitterionic dipalmitoylphosphatidylcholine (DPPC), and neutral cholesterol-and the pH of the subphase was varied between 3.5 and 6.0. In addition, the impact of the molecular weight of chitosan on the interactions was assessed at pH 3.5. It was found that while chitosan had a negligible effect on DPPC monolayers over the pH range studied, it distinctly affected DPPG and cholesterol monolayers. The effect on DPPG was found to decrease with increasing pH, that at pH 3.5 being ascribed to the charge-mediating action of chitosan on the local ionic environment and that at higher pHs to the intercalation of chitosan to the monolayers. Practically independent of pH, the effect of chitosan on cholesterol was accounted for by the formation of cholesterol-chitosan hydrogen bonds. Chitosan of lower molecular weight facilitated the interactions with all the three lipids studied. The results obtained may be helpful in identifying the mode of antibacterial activity of chitosan versus other modes that involve the disturbance of cell life cycles.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 055191480

Download citation: RISBibTeXText

PMID: 21936509

DOI: 10.1021/bm2012295


Related references

Probing chitosan and phospholipid interactions using Langmuir and Langmuir-Blodgett films as cell membrane models. Langmuir 23(14): 7666-7671, 2007

Molecular weight and pH effects of aminoethyl modified chitosan on antibacterial activity in vitro. International Journal of Biological Macromolecules 50(4): 918-924, 2012

Films of chitosan and chitosan-oligosaccharide neutralized and thermally treated: Effects on its antibacterial and other activities. Lebensmittel-Wissenschaft Und-Technologie / Food Science and Technology 73: 368-374, 2016

PH Effects on solubility, zeta potential, and correlation between antibacterial activity and molecular weight of chitosan. Carbohydrate Polymers 134: 74-81, 2015

Effect of molecular weight of chitosan on antimicrobial properties and tissue compatibility of chitosan-impregnated bacterial cellulose films. Biotechnology and Bioprocess Engineering 19(3): 534-544, 2014

In vitro Evaluation of Genipin-crosslinked Na-alginate/Chitosan Hydrogel Films for Delivery of Metformin: Effect of Chitosan Molecular Weight. Current Drug Delivery 15(8): 1146-1158, 2018

Effect of molecular weight, acid, and plasticizer on the physicochemical and antibacterial properties of β-chitosan based films. Journal of Food Science 77(5): E127-E136, 2012

Surface structure of chitosan and hybrid chitosan-amylose films-restoration of the antibacterial properties of chitosan in the amylose film. Carbohydrate Research 342(16): 2490-2493, 2007

Comparison in antioxidant action between α-chitosan and β-chitosan at a wide range of molecular weight and chitosan concentration. Bioorganic and Medicinal Chemistry 20(9): 2905-2911, 2012

The Effect of Molecular Weight on the Antibacterial Activity of N , N , N -Trimethyl Chitosan (TMC). International Journal of Molecular Sciences 20(7):, 2019

Regenerating activity and antibacterial effect of low-molecular-weight chitosan. BIOLOGY BULLETIN 32(6): 545-548, 2005

Regenerating activity and antibacterial effect of low-molecular-weight chitosan. Izvestiia Akademii Nauk. Seriia Biologicheskaia .(6): 659-663, 2006

Antimicrobial activity of a low-molecular-weight chitosan obtained from cellulase digestion of chitosan. Journal of Food Protection 67(2): 396-398, 2004

Antibacterial Activity of Alkylated and Acylated Derivatives of Low-Molecular Weight Chitosan. Prikladnaia Biokhimiia i Mikrobiologiia 52(2): 237-241, 2016

Antibacterial activity and mechanism of chitosan with ultra high molecular weight. Carbohydrate Polymers 148: 200-205, 2016