+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Prognostic significance of miR-218 in human hepatocellular carcinoma and its role in cell growth



Prognostic significance of miR-218 in human hepatocellular carcinoma and its role in cell growth



Oncology Reports 32(4): 1571-1577



MicroRNA-218 (miR-218) is considered a tumor suppressor in human cancer. In the present study, miR-218 expression was found to be significantly lower in human hepatocellular carcinoma (HCC) than in normal tumor-adjacent tissues. miR-218 was clearly silenced or downregulated in five HCC cells (HepG2, Hep3B, SMMC-7721, Huh7 and Bel-7402) compared with normal hepatocytes (LO2). The low expression of miR-218 conferred a poor 5-year survival in HCC patients. Multivariate Cox regression analysis indicated that miR-218 was an independent prognostic factor in HCC. Ectopic expression of miR-218 inhibited proliferation and promoted apoptosis in HepG2 and SMMC-7721 cells. In tumor bearing mice, miR-218 slowed down tumor growth by inducing apoptosis and growth arrest. Restoring miR-218 expression resulted in downregulation of B lymphoma Mo-MLV insertion region 1 homolog (BMI-1) mRNA and protein level in HepG2 and SMMC-7721 cells. In addition, BMI-1 mRNA expression in HCC was significantly higher than that in non-cancerous tissues. BMI-1 mRNA was inversely correlated with miR-218 expression in HCC tissues. In conclusion, miR-218 may serve as a prognostic biomarker and induce apoptosis and growth arrest by downregulating BMI-1 in HCC.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 055212712

Download citation: RISBibTeXText

PMID: 25110121

DOI: 10.3892/or.2014.3386


Related references

Knockdown of GTPBP4 inhibits cell growth and survival in human hepatocellular carcinoma and its prognostic significance. Oncotarget 8(55): 93984-93997, 2017

Prognostic role and significance of paraneoplastic syndromes in hepatocellular carcinoma. American Surgeon 80(2): 191-196, 2014

Prognostic significance of Bcl-xL in human hepatocellular carcinoma. Surgery 135(6): 604-612, 2004

Re: "Prognostic significance of Bcl-xL in human hepatocellular carcinoma". Surgery 137(2): 265-266, 2005

The molecular role of Fra-1 and its prognostic significance in human esophageal squamous cell carcinoma. Cancer 118(13): 3387-3396, 2012

The prognostic significance of macroscopic growth pattern of hepatocellular carcinoma. International Surgery 79(1): 38-42, 1994

Clinicopathological significance and prognostic role of p-STAT3 in patients with hepatocellular carcinoma. Oncotargets and Therapy 11: 1203-1214, 2018

Expression of Fra-1 in human hepatocellular carcinoma and its prognostic significance. Tumour Biology 39(6): 1010428317709635, 2017

Expression and prognostic significance of contactin 1 in human hepatocellular carcinoma. Oncotargets and Therapy 9: 387-394, 2016

Expression of serum miR-221 in human hepatocellular carcinoma and its prognostic significance. Biochemical and Biophysical Research Communications 406(1): 70-73, 2011

Expression of Kindlin-1 in human hepatocellular carcinoma and its prognostic significance. Tumour Biology 36(6): 4235-4241, 2015

Prognostic significance of natural killer cell infiltration in hepatocellular carcinoma. Ai Zheng 28(11): 1198-1202, 2009

Histologic characteristics and prognostic significance in small hepatocellular carcinoma with biliary differentiation: subdivision and comparison with ordinary hepatocellular carcinoma. American Journal of Surgical Pathology 31(5): 783-791, 2007

Expression of protein TARBP1 in human hepatocellular carcinoma and its prognostic significance. International Journal of Clinical and Experimental Pathology 8(8): 9089-9096, 2015

Prognostic significance of DNA topoisomerase IIalpha expression in human hepatocellular carcinoma.. Anticancer Research 22(2b): 1113-1119, 2002